GFP Antibody


Western Blot: GFP Antibody [NBP2-22220] - GFP expression in Cos-7 whole cell lysate using anti-GFP antibody. Cos-7 cells were transfected with an eGFP expression plasmid. Cells were lysed two days after transfection. more
Immunocytochemistry/ Immunofluorescence: GFP Antibody [NBP2-22220] - Confocal microscopy images of COS-7 cells transfected with expression constructs encoding membrane-tethered EGFP (membrane-EGFP; top) or nuclear more
Immunoprecipitation: GFP Antibody [NBP2-22220] - HEK293 cells using anti-GFP antibody. HEK293 cells were transfected with expression construct encoding GFP-NLS protein. Twenty hours post transfection cells were lysed more

Product Details

Reactivity JfSpecies Glossary
Applications WB, ICC/IF, IP
1 mg/ml

Order Details

GFP Antibody Summary

EGFP, a native full-length protein
Protein A purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
PBS, approx. pH 7.4
15mM Sodium Azide
1 mg/ml
Protein A purified


Application Notes
Western Blotting-Positive control: transfected cells-Negative control: non-transfected cells Immunocytochemistry-Positive control:transfected cells-Negative control:non-transfected cells
Reviewed Applications
Read 1 Review rated 5
NBP2-22220 in the following application:

Reactivity Notes

The polyclonal antibody recognizes GFP, EGFP, EYFP fusion proteins in Monkey and Human.

Alternate Names for GFP Antibody

  • eGFP
  • Enhanced Green Fluorescent Protein
  • GFPuv
  • green fluorescent protein (gfp)
  • Green Fluorescent Protein


Green fluorescence protein (GFP) is a 27 KDa protein derived from the bioluminiscent jellyfish Aquorea victoria, emiting green light (Lambda =509 nm) when excited (excitation by Blue or UV light, absorption peak Lambda =395 nm).GFP is a useful tool in cell biology research, as its intrinsic fluorescence can be visualized in living cells. Light-stimulated GFP fluorescence is species-independent and a fluorescence has been reported from many different types of GFP-expressing hosts, including microbes, invertebrates, vertebrates and plants. No exogenous substrates and cofactors are required for the fluorescence of GFP, since GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein.GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without any further staining. Other applications of GFP include measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. To increase a fluorescence intensity of GFP, chomophore mutations have been created. The EnhancedGFP has a fluorescence 35 times more intense than the wt-GFP. Mutagenesis of GFP has produced also many mutants (e.g. Yellow Fluorescent Protein, Cyan Fluorescent Protein) with warying spectral properties. Antibodies raised against full-length GFP variants should also detect other variants of the protein.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Reviews for GFP Antibody (NBP2-22220) (1) 51

Average Rating: 5
(Based on 1 reviews)
We have 1 review tested in 1 species: Other.
We have 1 review tested in 1 application: WB.

Reviews using NBP2-22220:
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Images Ratings Applications Species Date Details
Western Blot GFP NBP2-22220
reviewed by:
WB Other 01/22/2015


ApplicationWestern Blot
Sample TestedCos-7 whole cell lysate


Blocking DetailsBlocking Buffer: 5% Milk powder in TBS/0,1% Tween-20

Primary Anitbody

Dilution Ratio1:1000, 4°C overnight in blocking buffer

Secondary Antibody

Secondary Descriptiongoat anti-rabbit IgG POD
Secondary Manufacturer Cat#111-035-144
Secondary Concentration1:10000


Detection Notesenhanced Chemiluminescence, X-ray film, exporsure time: 2 minutes, lane 4: negative control, lanes3+5: eGFP-positive cells


CommentsCos-7 cells were transfected with an eGFP expression plasmid. Cells were lysed two days after transfection.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GFP Antibody (NBP2-22220). (Showing 1 - of FAQ).

Secondary Antibodies


Isotype Controls

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Bioinformatics Tool for GFP Antibody (NBP2-22220)

Discover related pathways, diseases and genes to GFP Antibody (NBP2-22220). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for GFP Antibody (NBP2-22220)

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Blogs on GFP.

GFP - Be Green!
Green fluorescence protein (GFP) is a 27KD protein derived from the jellyfish Aquorea victoria that emits a green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP is a highly v...  Read full blog post.

GFP (Green Fluorescent Protein) Bringing Light to Life
GFP (green florescent protein), originally discovered in the jellyfish Aequorea victoria, (1) is one of the most extensively investigated and exploited proteins in the area of life sciences. GFP is well known for its proficiency emit fluorescence and...  Read full blog post.

BDNF Antibodies Aid Research on Alzheimer's Therapies
Brain-derived neurotrophic factor (BDNF) is known to be important for neuronal differentiation, survival, migration and plasticity in both the developing embryo and adult synapses. The BDNF antibody is also proving to be an important tool in Alzheimer...  Read full blog post.

Epitope Tags and the V5 Tag Antibody
We at Novus Biologicals have a large antibody catalog targeted to epitope tags. They include C-Myc, GFP, HA, DYKDDDDK, and V5 Tag antibody reagents. The V5 tag antibody recognizes the artificially engineered V5 epitope, which is formed of amino acid r...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol GFP

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