GFP Antibody

Images

 
Western Blot: GFP Antibody [NBP2-22220] - GFP expression in Cos-7 whole cell lysate using anti-GFP antibody. Cos-7 cells were transfected with an eGFP expression plasmid. Cells were lysed two days after transfection. ...read more
Immunocytochemistry/ Immunofluorescence: GFP Antibody [NBP2-22220] - Confocal microscopy images of COS-7 cells transfected with expression constructs encoding membrane-tethered EGFP (membrane-EGFP; top) or nuclear ...read more
Immunoprecipitation: GFP Antibody [NBP2-22220] - HEK293 cells using anti-GFP antibody. HEK293 cells were transfected with expression construct encoding GFP-NLS protein. Twenty hours post transfection cells were lysed ...read more

Product Details

Summary
Product Discontinued
View other related GFP Primary Antibodies

Order Details


    • Catalog Number
      NBP2-22220
    • Availability
      Product Discontinued

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GFP Antibody Summary

Immunogen
EGFP, a native full-length protein
Clonality
Polyclonal
Host
Rabbit
Gene
GFP
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1.5ug/ml
  • Immunocytochemistry/Immunofluorescence 1-3ug/ml
  • Immunoprecipitation 10-20ug/Sample
Application Notes
Western Blotting-Positive control: transfected cells-Negative control: non-transfected cells Immunocytochemistry-Positive control:transfected cells-Negative control:non-transfected cells
Reviewed Applications
Read 3 Reviews rated 5
using
NBP2-22220 in the following applications:

Reactivity Notes

The polyclonal antibody recognizes GFP, EGFP, EYFP fusion proteins in Monkey and Human.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for GFP Antibody

  • eGFP
  • Enhanced Green Fluorescent Protein
  • GFP
  • GFPuv
  • green fluorescent protein (gfp)
  • Green Fluorescent Protein

Background

Green fluorescence protein (GFP) is a 27 KDa protein derived from the bioluminiscent jellyfish Aquorea victoria, emiting green light (Lambda =509 nm) when excited (excitation by Blue or UV light, absorption peak Lambda =395 nm).GFP is a useful tool in cell biology research, as its intrinsic fluorescence can be visualized in living cells. Light-stimulated GFP fluorescence is species-independent and a fluorescence has been reported from many different types of GFP-expressing hosts, including microbes, invertebrates, vertebrates and plants. No exogenous substrates and cofactors are required for the fluorescence of GFP, since GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein.GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without any further staining. Other applications of GFP include measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. To increase a fluorescence intensity of GFP, chomophore mutations have been created. The EnhancedGFP has a fluorescence 35 times more intense than the wt-GFP. Mutagenesis of GFP has produced also many mutants (e.g. Yellow Fluorescent Protein, Cyan Fluorescent Protein) with warying spectral properties. Antibodies raised against full-length GFP variants should also detect other variants of the protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GFP Antibody (NBP2-22220) (0)

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Reviews for GFP Antibody (NBP2-22220) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Other.
We have 3 reviews tested in 2 application: ICC, WB.

Reviews using NBP2-22220:
Filter by Applications
ICC
(1)
WB
(2)
All Applications
Filter by Species
Other
(3)
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Images Ratings Applications Species Date Details
Immunocytochemistry GFP NBP2-22220
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reviewed by:
Anonymous
ICC Other 01/16/2017
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Summary

ApplicationImmunocytochemistry
Sample TestedGFP expressing pig TM cells
SpeciesOther

Comments

CommentsPig TM cells express GFP
Plenti CMV EGFP DEST plasmid and lenti packaging plasmids were transfected to HEK 293 cells, after two days lenti virus was produced and transduced to pig TM cells for 2-3 days.
Western Blot GFP NBP2-22220
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reviewed by:
Anonymous
WB Other 01/04/2017
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Summary

ApplicationWestern Blot
Sample TestedPig TM cells transduced with GFP
SpeciesOther

Comments

CommentsWB blot showing detection of GFP (red color) using GFP antibody (NBP2-22220) at 1:2000 dilution and the cell lysates from pig TM cells transduced with lenti virus carrying GFP
Western Blot GFP NBP2-22220
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reviewed by:
Anonymous
WB Other 01/22/2015
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Summary

ApplicationWestern Blot
Sample TestedCos-7 whole cell lysate
SpeciesOther
Lot517777

Blocking

Blocking DetailsBlocking Buffer: 5% Milk powder in TBS/0,1% Tween-20

Primary Anitbody

Dilution Ratio1:1000, 4°C overnight in blocking buffer

Secondary Antibody

Secondary Descriptiongoat anti-rabbit IgG POD
Secondary Manufacturer Cat#111-035-144
Secondary Concentration1:10000

Details

Detection Notesenhanced Chemiluminescence, X-ray film, exporsure time: 2 minutes, lane 4: negative control, lanes3+5: eGFP-positive cells

Comments

CommentsCos-7 cells were transfected with an eGFP expression plasmid. Cells were lysed two days after transfection.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GFP Antibody (NBP2-22220). (Showing 1 - of FAQ).

Secondary Antibodies

 

Isotype Controls

Additional GFP Products

Bioinformatics Tool for GFP Antibody (NBP2-22220)

Discover related pathways, diseases and genes to GFP Antibody (NBP2-22220). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for GFP Antibody (NBP2-22220)

Find related products by research area.

Blogs on GFP.

The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

GFP - Be Green!
Green fluorescence protein (GFP) is a 27KD protein derived from the jellyfish Aquorea victoria that emits a green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP is a highly v...  Read full blog post.

GFP (Green Fluorescent Protein) Bringing Light to Life
GFP (green florescent protein), originally discovered in the jellyfish Aequorea victoria, (1) is one of the most extensively investigated and exploited proteins in the area of life sciences. GFP is well known for its proficiency emit fluorescence and...  Read full blog post.

BDNF Antibodies Aid Research on Alzheimer's Therapies
Brain-derived neurotrophic factor (BDNF) is known to be important for neuronal differentiation, survival, migration and plasticity in both the developing embryo and adult synapses. The BDNF antibody is also proving to be an important tool in Alzheimer...  Read full blog post.

Epitope Tags and the V5 Tag Antibody
We at Novus Biologicals have a large antibody catalog targeted to epitope tags. They include C-Myc, GFP, HA, DYKDDDDK, and V5 Tag antibody reagents. The V5 tag antibody recognizes the artificially engineered V5 epitope, which is formed of amino acid r...  Read full blog post.

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Calculators

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Recent Reviews

5
5
3
4
0
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0
2
0
1
0

Anonymous
01/16/2017
Application: ICC
Species: Other

Anonymous
01/04/2017
Application: WB
Species: Other

Anonymous
01/22/2015
Application: WB
Species: Other

Bioinformatics

Gene Symbol GFP