Western Blot: GFP Antibody [NBP2-22220] - GFP expression in Cos-7 whole cell lysate using anti-GFP antibody. Cos-7 cells were transfected with an eGFP expression plasmid. Cells were lysed two days after transfection. ...read more
Immunocytochemistry/ Immunofluorescence: GFP Antibody [NBP2-22220] - Confocal microscopy images of COS-7 cells transfected with expression constructs encoding membrane-tethered EGFP (membrane-EGFP; top) or nuclear ...read more
Immunoprecipitation: GFP Antibody [NBP2-22220] - HEK293 cells using anti-GFP antibody. HEK293 cells were transfected with expression construct encoding GFP-NLS protein. Twenty hours post transfection cells were lysed ...read more
The polyclonal antibody recognizes GFP, EGFP, EYFP fusion proteins in Monkey and Human.
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
PBS (pH 7.4)
15mM Sodium Azide
Protein A purified
Alternate Names for GFP Antibody
Enhanced Green Fluorescent Protein
green fluorescent protein (gfp)
Green Fluorescent Protein
Green fluorescence protein (GFP) is a 27 KDa protein derived from the bioluminiscent jellyfish Aquorea victoria, emiting green light (Lambda =509 nm) when excited (excitation by Blue or UV light, absorption peak Lambda =395 nm).GFP is a useful tool in cell biology research, as its intrinsic fluorescence can be visualized in living cells. Light-stimulated GFP fluorescence is species-independent and a fluorescence has been reported from many different types of GFP-expressing hosts, including microbes, invertebrates, vertebrates and plants. No exogenous substrates and cofactors are required for the fluorescence of GFP, since GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein.GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without any further staining. Other applications of GFP include measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. To increase a fluorescence intensity of GFP, chomophore mutations have been created. The EnhancedGFP has a fluorescence 35 times more intense than the wt-GFP. Mutagenesis of GFP has produced also many mutants (e.g. Yellow Fluorescent Protein, Cyan Fluorescent Protein) with warying spectral properties. Antibodies raised against full-length GFP variants should also detect other variants of the protein.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Pig TM cells express GFP Plenti CMV EGFP DEST plasmid and lenti packaging plasmids were transfected to HEK 293 cells, after two days lenti virus was produced and transduced to pig TM cells for 2-3 days.
GFP - Be Green! Green fluorescence protein (GFP) is a 27KD protein derived from the jellyfish Aquorea victoria that emits a green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP is a highly v... Read full blog post.
Epitope Tags and the V5 Tag Antibody We at Novus Biologicals have a large antibody catalog targeted to epitope tags. They include C-Myc, GFP, HA, DYKDDDDK, and V5 Tag antibody reagents. The V5 tag antibody recognizes the artificially engineered V5 epitope, which is formed of amino acid r... Read full blog post.
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