GFP Antibody (454505) [Alexa Fluor® 594]

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HEK293 human embryonic kidney cell line transfected with eGFP were stained with and either (A) Mouse Anti-GFP Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # FAB42401T) or (B) Mouse IgG1 ...read more

Product Details

Summary
Reactivity MultiSpecies Glossary
Applications Flow
Clone
454505
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 594
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

GFP Antibody (454505) [Alexa Fluor® 594] Summary

Immunogen
E. coli-derived recombinant GFPuv
Ser2-Lys238
Accession # P42212
Specificity
Detects recombinant GFPuv and eGFP in Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GFP
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GFP Antibody (454505) [Alexa Fluor® 594]

  • eGFP
  • Enhanced Green Fluorescent Protein
  • GFP
  • GFPuv
  • green fluorescent protein (gfp)
  • Green Fluorescent Protein

Background

Green fluorescent protein (GFP) is a 27 kDa protein originally isolated from the jellyfish Aequorea victoria. In the presence of UV light (490-520 nm), it emits a green fluorescent color that can be used to pinpoint locations of various intracellular proteins. GFP is 238 amino acids (aa) in length. It is a globular monomer that has a tendency to dimerize. The monomer has the shape of a beta -barrel with a chromophore (aa 65-67) containing alpha -helix running up its center. GFPuv is the Aequorea sequence with three aa substitutions; Phe to Ser at # 99, Met to Thr at # 153, and Val to Ala at # 163. This form expresses faster and is 18-fold brighter than native GFP; excitation peaks at 395 nm and emission at 508 nm.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for GFP Antibody (FAB42401T). (Showing 1 - of FAQ).

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    GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

    GFP - Be Green!
    Green fluorescence protein (GFP) is a 27KD protein derived from the jellyfish Aquorea victoria that emits a green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP is a highly v...  Read full blog post.

    GFP (Green Fluorescent Protein) Bringing Light to Life
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    Bioinformatics

    Gene Symbol GFP
    Uniprot