GDPD2 Antibody (501016)

GDPD2 was detected in immersion fixed MC3T3-E1 mouse preosteoblast cell line using Mouse Anti-Human GDPD2 Monoclonal Antibody (Catalog # MAB7026) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoskeleton. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu, Mu
• Applications: ICC/IF
• Clone: 501016
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

GDPD2 Antibody (501016) Summary

• Immunogen: HEK293 human embryonic kidney cell line transfected with human GDPD2
  Accession # Q9HCC8
• Specificity: Detects human and mouse GDPD2.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: GDPD2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GDPD2 Antibody (501016)

• EC 3.1.4.43
• FLJ20207
• GDE3
• GDE3Osteoblast differentiation promoting factor
• GDPD2
• Glycerophosphodiester phosphodiesterase 3
• glycerophosphodiester phosphodiesterase domain containing 2
• Glycerophosphodiester phosphodiesterase domain-containing protein 2
• OBDPF
• OBDPFglycerophosphoinositol inositolphosphodiesterase GDPD2

Background

Glycerophosphodiester phosphodiesterase 3 (GDPD2), also known as GDE3, is a 65 kDa 7TM cell surface protein that is highly expressed in bone and spleen. GDPD2 promotes osteoblast differentiation and induces changes in the actin cytoskeleton. Its 225 amino acid (aa) N-terminal ECD contains a domain that is homologous to bacterial glycerophosphodiester phosphodiesterases. Human GDPD2 shares 85% aa sequence identity with mouse and rat GDPD2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GDPD2
• Uniprot:
  • Q9HCC8()