![Immunocytochemistry/Immunofluorescence: GCN4 Antibody (C11L34) [NBP2-81273] - Immunofluorescence staining of fixed HeLa cells expressing mCherry tagged GCN4 with anti-GCN4 antibody (C11L34) [NBP2-81273]. Immunofluorescence analysis of fixed HeLa cells stained with the chimeric mouse IgG1 version of C11L34 [NBP2-81273] followed by an Alexa Fluor 488 secondary antibody, showing mitochondrial staining. Colocalisation with mCherry tagged GCN4 demonstrates the very low off target/non-specific binding of this antibody. The nuclear and mitochondiral DNA stain is DAPI (blue). Panels show; a) mCherry tagged, mitochondrially targetted GCN4, b) NBP2-81273 with Alexa Fluor 488 secondary antibody, c) DNA stained with DAPI, d) Merged channels. Images courtesy of Luming Chen (Kim Orth lab, UT Southwestern Medical Center).](http://images.novusbio.com/fullsize/GCN4-Antibody-C11L34-Immunocytochemistry-Immunofluorescence-NBP2-81273-img0002.jpg "Immunocytochemistry/Immunofluorescence: GCN4 Antibody (C11L34) [NBP2-81273] - Immunofluorescence staining of fixed HeLa cells expressing mCherry tagged GCN4 with anti-GCN4 antibody (C11L34) [NBP2-81273]. Immunofluorescence analysis of fixed HeLa cells stained with the chimeric mouse IgG1 version of C11L34 [NBP2-81273] followed by an Alexa Fluor 488 secondary antibody, showing mitochondrial staining. Colocalisation with mCherry tagged GCN4 demonstrates the very low off target/non-specific binding of this antibody. The nuclear and mitochondiral DNA stain is DAPI (blue). Panels show; a) mCherry tagged, mitochondrially targetted GCN4, b) NBP2-81273 with Alexa Fluor 488 secondary antibody, c) DNA stained with DAPI, d) Merged channels. Images courtesy of Luming Chen (Kim Orth lab, UT Southwestern Medical Center).")
| Reactivity | YeSpecies Glossary |
| Applications | WB, ICC/IF, IP, SPR |
| Clone | C11L34 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Biotinylated GCN4-peptide (HLENEVARLKK). |
| Specificity | This antibody binds to the HLENEVARLKK peptide from the leucine-zipper domain of the S. cerevisiae GCN4 transcriptional activator with picomolar affinity. |
| Isotype | IgG1 Lambda |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | GCN4 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This full-length, reformatted mouse antibody was made using the variable domain sequences of the original Mouse scFv format, for improved compatibility with existing reagents, assays and techniques. This antibody binds to the GCN4-leucine zipper peptide, which is part of the dimerisation- and DNA-binding domain of the transcriptional activator GCN4 in S. cerevisiae. |
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| Storage | Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C. |
| Buffer | PBS |
| Preservative | 0.02% Proclin 300 |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
| Publications using NBP2-81273 | Applications | Species |
|---|---|---|
| Alecki C, Rizwan J, Le P et al. Localized synthesis of molecular chaperones sustains neuronal proteostasis bioRxiv : the preprint server for biology 2023-10-03 [PMID: 37873158] (Immunocytochemistry/ Immunofluorescence) | Immunocytochemistry/ Immunofluorescence | |
| Chen R, Stainier W, Dufourt J et al. Repressor sequestration activates translation of germ granule localized mRNA bioRxiv 2023-10-18 (Immunocytochemistry/ Immunofluorescence) | Immunocytochemistry/ Immunofluorescence |
Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry-Paraffin: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] [NB7539] - FFPE serial sections of human stomach carcinoma. Primary Antibody: Mouse anti-p53 (Clone DO-1) used at a dilution of 1:100. Secondary Antibody: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] Conjugated used at a dilution of 1:200 (5ug/ml). Detection: DAB](https://images.novusbio.com/images/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-HRP-Immunohistochemistry-Paraffin-NB7539-img0014.jpg "Immunohistochemistry-Paraffin: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] [NB7539] - FFPE serial sections of human stomach carcinoma. Primary Antibody: Mouse anti-p53 (Clone DO-1) used at a dilution of 1:100. Secondary Antibody: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] Conjugated used at a dilution of 1:200 (5ug/ml). Detection: DAB")
