Gag-Pro-Pol polyprotein Antibody

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Western Blot: Gag-Pro-Pol polyprotein Antibody [NBP2-44178] - lane 1: cell lysate negative control. Lane 2: cell lysate spiked with purified virus. Load: 10 Ug per lane.

Product Details

Summary
Reactivity V-ViSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Gag-Pro-Pol polyprotein Antibody Summary

Immunogen
Gag-Pro-Pol polyprotein Antibody was prepared from whole rabbit serum produced by repeated immunizations with a full length sequence for mouse mammary tumor virus capsid protein tagged with His. (Uniprot: P11283)
Isotype
Serum
Clonality
Polyclonal
Host
Rabbit
Purity
Delipidation and Defibrination
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Applications/Dilutions

Dilutions
  • ELISA 1:10000-1:20000
  • Western Blot 1:1000-1:2000
Application Notes
This product has been tested for use in western blotting. Specific conditions for reactivity should be optimized by the end user. Expect band approximately 26.7 kDa in size corresponding to MMTV capsid protein by western blotting in the appropriate cell lysate or extract.

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with Gag-Pro-Pol polyprotein from mouse based on a 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Packaging, Storage & Formulations

Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Delipidation and Defibrination

Notes

Mouse mammary tumor virus capsid antibody was prepared from monospecific, delipidated and defibrinated antiserum, with addition of sodium azide to 0.01% and cross adsorbed with 6X HIS

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for Gag-Pro-Pol polyprotein Antibody

  • Capsid protein p27
  • Gag-Pro-Pol
  • MMTV capsid
  • Mouse mammary tumor virus (strain C3H)
  • Reverse transcriptase/ribonuclease H

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Uniprot