Fructosyl amino acid oxidase Antibody [Biotin]

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Order Details


    • Catalog Number
      NBP1-77716
    • Availability
      Product Discontinued

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Fructosyl amino acid oxidase Antibody [Biotin] Summary

Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Sheep Serum as well as purified and partially purified Fructosyl Amino Acid Oxidase [E.coli]
Immunogen
Fructosyl amino acid oxidase [from Corynebacterium sp. Expressed in E.coli] (Uniprot: Q8RIU8)
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Purity
Multi-step
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Applications/Dilutions

Dilutions
  • ELISA 1:5000 - 1:20000
  • Immunoprecipitation 1:100
  • Western Blot 1:500 - 1:5000
Application Notes
This product has been assayed against 1.0 ug of Fructosyl Amino Acid Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:8,000 to 1:34,000 of the reconstitution concentration is suggested for this product.
Readout System

Reactivity Notes

Cross reactivity against Fructosyl amino acid oxidase from other sources is unknown.

Packaging, Storage & Formulations

Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Multi-step
Reconstitution Instructions
Reconstitute with 100 ul deionized water (or equivalent)

Alternate Names for Fructosyl amino acid oxidase Antibody [Biotin]

  • EC=1.5.3.-
  • FAOX-C

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Uniprot