FOLR4 Antibody (TH6)

Flow Cytometry: FOLR4 Antibody (TH6) [NBP2-26651] - Analysis using the PE conjugate of NBP2-26657. Staining using folate receptor 4 antibody. CD4/25+ gated mouse splenocytes probed using rat IgG2b isotype control antibody (left) and folate receptor 4 antibody (right) at 0.1 ug/10^6 cells.

Flow Cytometry: FOLR4 Antibody (TH6) [NBP2-26651] - Analysis of FITC conjugate of NBP2-26651. Analysis using folate receptor 4 antibody. Tri-color staining of BALB/c splenocytes for FR4 FITC (NBP2-26659 and NBP2-26660 used at 0.06 ug/10^6 cells), CD4 PE and CD25 APC. CD4+/25+, CD4+/25-, and CD4- cells were

Flow Cytometry: FOLR4 Antibody (TH6) [NBP2-26651] - Analysis using Azide Free version of NBP2-26651. Analysis using folate receptor 4 antibody. CD4/25+ gated mouse splenocytes probed using A) rat IgG2b isotype control antibody and B) folate receptor 4 antibody at 0.06 ug/10^6 cells.

Flow Cytometry: FOLR4 Antibody (TH6) [NBP2-26651] - Analysis using the FITC conjugate of NBP2-26657. Staining using folate receptor 4 antibody. Staining of intracellular FoxP3 and cell surface folate receptor 4 with clone 12A5 (left) and with clone (right) at 0.06 ug/10^6 mouse splenocytes.

Flow Cytometry: FOLR4 Antibody (TH6) [NBP2-26651] - Analysis using the PE conjugate of NBP2-26657. Staining using folate receptor 4 antibody. CD4/25+ gated mouse splenocytes probed using rat IgG2b isotype control antibody (left) and folate receptor 4 antibody (right) at 0.1 ug/10^6 cells.

Flow Cytometry: FOLR4 Antibody (TH6) [NBP2-26651] - Analysis using the Allophycocyanin conjugate of NBP2-26657. Staining using FOLR4 antibody. CD25+ gated mouse splenocytes probed using rat IgG2b isotype control antibody (left) and folate receptor 4 antibody (right) at 0.015 ug/10^6 cells.

Product Discontinued

FOLR4 Antibody (TH6) Summary

• Immunogen: CD3 mAb-stimulated CD4/25 T cells
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: IZUMO1R
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.05% BSA.
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for FOLR4 Antibody (TH6)

• folate receptor 4 (delta) homolog (mouse)
• Folbp3
• FOLR4
• FR-delta
• LOC390243
• probable folate receptor delta

Background

Folates represent an important role in protein and nucleic acid biosynthesis and are recognized in particular for their role in spinal canal and brain development during early pregnancy. Recently, Yamaguchi et al, 2007 have shown that Folate receptor 4 (FR4), a subtype of the receptor for the vitamin folic acid is expressed at high levels in both natural and TGF-b-induced Treg cells. A majority of the CD4+ Tregs express Folate receptor 4 along with CD25 and FoxP3. In addition, there are functional similarities in regulating immune response by FoxP3 and FR4 expressing CD4+ Tregs which express CD25 as well. CD4+/25+FR4hi T cell population may be used as a functional immuno-suppressor Treg population which should express FoxP3. Folate receptor 4 may be considered as a surface marker for Tregs. The antibody produced by clones 12A5 and TH6 recognize FR4 in Tregs. FR4 is also known as folate receptor delta and folate-binding protein 3. FR4 antibody in combination with CD4/25 antibody may be used for isolation (both FACS and magnetic beads sorting) and staining of Tregs using simple surface staining while keeping the Tregs viable for subsequent down stream applications. Folate receptor 4 is known to be expressed exclusively in lymphoid tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FOLR4 Antibody (NBP2-26651)(1)

Publication using NBP2-26651

• Castro FV, Al-Muftah M, Mulryan K et al Regulation of autologous immunity to the mouse 5T4 oncofoetal antigen: implications for immunotherapy. Cancer Immunol Immunother. 2012-07-01 [PMID: 22127365]
  
  Details:
  Citation using the Azide Free version of this antibody.

Bioinformatics

• Gene Symbol: IZUMO1R