FOLR4 Antibody (721601) [Alexa Fluor® 405]

• Reactivity: Mu
• Applications: WB
• Clone: 721601
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 405

FOLR4 Antibody (721601) [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant mouse FOLR4
  Gly20-Ser100
  Accession # Q9EQF4
• Specificity: Detects mouse FOLR4 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) FOLR1, rmFOLR2, recombinant human (rh) FOLR3, or rhFOLR4 is observed. In Western blots, no cross-reactivity with rhFOLR1, 2, or 3 is
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FOLR4 Antibody (721601) [Alexa Fluor® 405]

• folate receptor 4 (delta) homolog (mouse)
• Folbp3
• FOLR4
• FR-delta
• LOC390243
• probable folate receptor delta

Background

FOLR4 (folate receptor 4; also FR4, FR-d and FOLBP-3) is a 35 kDa glycoprotein member of the folate receptor family. It is found on mouse Treg cells, particularly those classified as natural, and CD4⁺ CD25^- T cells that produce low amounts of IFN-gamma . Although folate family receptors can internalize folate, they are generally not the principal conduits for folate uptake. Mouse FOLR4 preproprecursor is 244 amino acids (aa) in length. It contains a 19 aa signal sequence plus a 225 aa proform. While there are no definitive structural motifs, there is presumably a C-terminal propeptide that gives rise to a GPI-linkage. There are three potential isoforms. One is 37 kDa in size and shows a 36 aa insertion after Glu155. Two others show a deletion of  aa 47-109, and a 17 aa substitution for aa 157-244. Over aa 20-100, mouse FOLR4 shares 84% and 70% aa identity with rat and human FOLR4, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.