FOLR4 Antibody (12A5) [FITC]

Flow Cytometry: FOLR4 Antibody (12A5) [FITC] [NBP2-26660] - Analysis of intracellular FoxP3 and cell surface FR4 with clone 12A5 (left) and with clone TH6 (right) at 0.06 ug/10^6 mouse splenocytes.

Flow Cytometry: FOLR4 Antibody (12A5) [FITC] [NBP2-26660] - Tri-color staining of BALB/c splenocytes for FR4 FITC used at 0.06 ug/10^6 cells), CD4 PE (BD) and CD25 APC. CD4+CD25+, CD4+CD25-, and CD4- cells were analyzed for CD4 and FR4 expression by dot-plot flow cytometry. Rat IgG2b, k FITC was used as the isotype control.

Flow Cytometry: FOLR4 Antibody (12A5) [FITC] [NBP2-26660] - Tri-color staining of BALB/c splenocytes for FR4 FITC CD4 PE (BD) and CD25 APC. CD4+CD25+, CD4+CD25- and CD4- cells were analyzed for FR4 expression by flow cytometry. Rat IgG2b, k FITC was used as the isotype control.

Product Discontinued

FOLR4 Antibody (12A5) [FITC] Summary

• Immunogen: CD3 mAb-stimulated CD4/CD25 T cells
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: IZUMO1R
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1ul/1 million cells

Reactivity Notes

Mouse

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for FOLR4 Antibody (12A5) [FITC]

• folate receptor 4 (delta) homolog (mouse)
• Folbp3
• FOLR4
• FR-delta
• LOC390243
• probable folate receptor delta

Background

Folates represent an important role in protein and nucleic acid biosynthesis and are recognized in particular for their role in spinal canal and brain development during early pregnancy. Recently, Yamaguchi et al, 2007 have shown that Folate receptor 4 (FR4), a subtype of the receptor for the vitamin folic acid is expressed at high levels in both natural and TGF-b-induced Treg cells. A majority of the CD4+ Tregs express FR4 along with CD25 and FoxP3. In addition, there are functional similarities in regulating immune response by FoxP3 and FR4 expressing CD4+ Tregs which express CD25 as well. CD4+CD25+FR4hi T cell population may be used as a functional immuno-suppressor Treg population which should express FoxP3. FR4 may be considered as a cell surface marker for Tregs. The antibodies produced by clones 12A5 and TH6 recognize FR4 in Tregs. FR4 is also known as folate receptor delta and folate-binding protein 3. The FR4 antibodies in combination with CD4 and CD25 antibodies may be used for isolation (both FACS and magnetic beads sorting) and staining of Tregs using simple surface staining while keeping the Tregs viable for subsequent down stream applications. FR4 is known to be expressed exclusively in lymphoid tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IZUMO1R