FOLR4 Antibody (12A5) [FITC]


Flow Cytometry: FOLR4 Antibody (12A5) [FITC] [NBP2-26660] - Analysis of intracellular FoxP3 and cell surface FR4 with clone 12A5 (left) and with clone TH6 (right) at 0.06 ug/10^6 mouse splenocytes.
Flow Cytometry: FOLR4 Antibody (12A5) [FITC] [NBP2-26660] - Tri-color staining of BALB/c splenocytes for FR4 FITC used at 0.06 ug/10^6 cells), CD4 PE (BD) and CD25 APC. CD4+CD25+, CD4+CD25-, and CD4- cells were analyzed more
Flow Cytometry: FOLR4 Antibody (12A5) [FITC] [NBP2-26660] - Tri-color staining of BALB/c splenocytes for FR4 FITC CD4 PE (BD) and CD25 APC. CD4+CD25+, CD4+CD25- and CD4- cells were analyzed for FR4 expression by flow more

Product Details

Reactivity MuSpecies Glossary
Applications Flow
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FOLR4 Antibody (12A5) [FITC] Summary

CD3 mAb-stimulated CD4/CD25 T cells
IgG2b Kappa
Protein G purified
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Packaging, Storage & Formulations

Store at 4C in the dark.
FITC (A=495, E=519)
50mM Sodium Borate
0.05% Sodium Azide
Protein G purified


  • Flow Cytometry 1ul/1 million cells

Reactivity Notes


Alternate Names for FOLR4 Antibody (12A5) [FITC]

  • folate receptor 4 (delta) homolog (mouse)
  • Folbp3
  • FR-delta
  • LOC390243
  • probable folate receptor delta


Folates represent an important role in protein and nucleic acid biosynthesis and are recognized in particular for their role in spinal canal and brain development during early pregnancy. Recently, Yamaguchi et al, 2007 have shown that Folate receptor 4 (FR4), a subtype of the receptor for the vitamin folic acid is expressed at high levels in both natural and TGF-b-induced Treg cells. A majority of the CD4+ Tregs express FR4 along with CD25 and FoxP3. In addition, there are functional similarities in regulating immune response by FoxP3 and FR4 expressing CD4+ Tregs which express CD25 as well. CD4+CD25+FR4hi T cell population may be used as a functional immuno-suppressor Treg population which should express FoxP3. FR4 may be considered as a cell surface marker for Tregs. The antibodies produced by clones 12A5 and TH6 recognize FR4 in Tregs. FR4 is also known as folate receptor delta and folate-binding protein 3. The FR4 antibodies in combination with CD4 and CD25 antibodies may be used for isolation (both FACS and magnetic beads sorting) and staining of Tregs using simple surface staining while keeping the Tregs viable for subsequent down stream applications. FR4 is known to be expressed exclusively in lymphoid tissues.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies


Isotype Controls

Other Available Formats

Allophycocyanin Labeled NBP2-26666
Biotin Labeled NBP2-26658B
DyLight 350 Labeled NBP2-26658UV
DyLight 405 Labeled NBP2-26658V
DyLight 405LS Labeled NBP2-26658V3
DyLight 488 Labeled NBP2-26658G
DyLight 550 Labeled NBP2-26658R
DyLight 680 Labeled NBP2-26658FR
HRP Labeled NBP2-26658H
PE Labeled NBP2-26663
PerCP Labeled NBP2-26658PCP

Additional FOLR4 Antibody (12A5) [FITC] Products

FOLR4 NBP2-26660

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Gene Symbol FOLR4

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