![Western Blot: Flp recombinase Antibody [NBP3-11867] - Western blot was performed on whole cell lysates from untransfected 293 cells (lane 1), or 293 cells transfected with Cre (lane 2), Dre (lane 3) or Flp (lane 4) with NBP3-11867 diluted 1:500 in BSA/PBS-Tween. The molecular weight marker (in KD) is shown on the left; the location of the protein of interest (expected size: 48 KD) is indicated on the right.](http://images.novusbio.com/fullsize/Flp-recombinase-Antibody-Western-Blot-NBP3-11867-img0002.jpg "Western Blot: Flp recombinase Antibody [NBP3-11867] - Western blot was performed on whole cell lysates from untransfected 293 cells (lane 1), or 293 cells transfected with Cre (lane 2), Dre (lane 3) or Flp (lane 4) with NBP3-11867 diluted 1:500 in BSA/PBS-Tween. The molecular weight marker (in KD) is shown on the left; the location of the protein of interest (expected size: 48 KD) is indicated on the right.")
| Reactivity | All-NASpecies Glossary |
| Applications | WB, ELISA |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Novus Biologicals Rabbit Flp recombinase Antibody (NBP3-11867) is a polyclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Polyclonal antibody raised in rabbit against Flp recombinase using 3 KLH-conjugated synthetic peptides located at the N-terminal part of the protein (Uniprot#: P03870) |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Whole Antiserum |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles. |
| Buffer | Whole rabbit antiserum |
| Preservative | 0.05% Sodium Azide |
| Purity | Whole Antiserum |
Secondary Antibodies |
Isotype Controls |
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![ELISA: Flp recombinase Antibody [NBP3-11867] - To determine the titer, an ELISA was performed using a serial dilution of NBP3-11867 in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:9,000.](http://images.novusbio.com/fullsize/Flp-recombinase-Antibody-ELISA-NBP3-11867-img0001.jpg "ELISA: Flp recombinase Antibody [NBP3-11867] - To determine the titer, an ELISA was performed using a serial dilution of NBP3-11867 in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:9,000.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
