FITC Antibody (LO-FLUO-1) [Biotin]

Product Discontinued

FITC Antibody (LO-FLUO-1) [Biotin] Summary

• Immunogen: FITC conjugated mouse immunoglobulin
• Specificity: Binds to both free FITC and FITC conjugated to protein such as antibodies. The reagent may be useful for amplification of staining using FITC conjugated reagents.
• Isotype: IgM
• Clonality: Monoclonal
• Host: Rat
• Purity: IgM purified

Applications/Dilutions

• Dilutions:
  • ELISA 1.25 - 2.25 ug / ml
  • Flow Cytometry 1:10-1:1000
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Reactivity Notes

Reacts with Chemical.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: IgM purified

Alternate Names for FITC Antibody (LO-FLUO-1) [Biotin]

• FITC
• Fluorescein Isothiocyanate

Background

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.