Fibroblast Antibody (ER-TR7) - Azide and BSA Free

Immunohistochemistry: Fibroblast Antibody (ER-TR7) - Azide and BSA Free [NBP2-80736] - Fibroblast Antibody (ER-TR7) IHC on Mouse lymph node, frozen section, 20x. Primary antibody diluted 1:500. This image was submitted via customer Review. Image from the standard format of this antibody.

• Reactivity: Mu
• Applications: Flow, ICC/IF, IHC, CyTOF-ready
• Clone: ER-TR7
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Fibroblast Antibody (ER-TR7) - Azide and BSA Free Summary

• Description: Novus Biologicals Rat Fibroblast Antibody (ER-TR7) - Azide and BSA Free (NB100-64932) is a monoclonal antibody validated for use in IHC, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Isolated C3H thymic stromal cells
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry reported in scientific literature (PMID 22042977)
  • Immunohistochemistry-Frozen 1:500
• Application Notes: Membrane permeabilisation is required for Flow Cytometry.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Fibroblast Antibody (ER-TR7) - Azide and BSA Free

• reticular fibroblast

Background

Connective tissue consists of a ground of glycosaminoglycans, proteoglycans and glycoproteins through which various fibres run. These fibres can be collagenous, elastic or reticular. Reticular fibres are composed from the family of collagen proteins and give tensile strength. These fibres are made by reticular fibroblasts. This monoclonal antibody [ER-TR7] detects an antigen present in, and produced by, reticular fibroblasts. The antibody is useful to stain the microanatomy of various organs, in particular the connective tissue framework in lymphoid organs. The antibody also stains subendothelial deposits in the plaque area of atherosclerotic plaques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.