Fibroblast Antibody (ER-TR7)

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-Fr
Clone
ER-TR7
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Fibroblast Antibody (ER-TR7) Summary

Immunogen
Isolated C3H thymic stromal cells
Marker
Fibroblast Marker
Specificity
NB100-64932 recognizes ER-TR7, an antigen that is located in the cytoplasm of reticular fibroblasts and is a component of the extracellular matrix of lymphoid and non-lymphoid organs. The antigen recognized by clone ER-TR7 has not been identified but studies suggest that it is likely to be distinct from laminin, fibronectin, collagen types I-IV, heparin sulphate proteoglycan, entactin and nidogen. Clone ER-TR7 has been used to stain the microanatomy of various organs and also stains subendothelial deposits in atherosclerotic plaques.
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:50-1:100
Application Notes
Membrane permeabilisation is required for Flow Cytometry. Use in Immunohistochemistry has been reported in the literature: (PMID: 22042977)
Reviewed Applications
Read 1 Review rated 4
using
NB100-64932 in the following applications:

Publications
Read Publications using
NB100-64932 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Phosphate buffered saline
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Fibroblast Antibody (ER-TR7)

  • reticular fibroblast

Background

Connective tissue consists of a ground of glycosaminoglycans, proteoglycans and glycoproteins through which various fibres run. These fibres can be collagenous, elastic or reticular. Reticular fibres are composed from the family of collagen proteins and give tensile strength. These fibres are made by reticular fibroblasts. This monoclonal antibody [ER-TR7] detects an antigen present in, and produced by, reticular fibroblasts. The antibody is useful to stain the microanatomy of various organs, in particular the connective tissue framework in lymphoid organs. The antibody also stains subendothelial deposits in the plaque area of atherosclerotic plaques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fibroblast Antibody (NB100-64932)(6)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: FLOW, Flow, ICC/IF, IHC.


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FLOW
(1)
Flow
(1)
ICC/IF
(2)
IHC
(2)
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Mouse
(4)
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Showing Publications 1 - 6 of 6.
Publications using NB100-64932 Applications Species
Konda N, Saeki N, Nishino S, Ogawa K. Truncated EphA2 likely potentiates cell adhesion via integrins as well as infiltration and/or lodgment of a monocyte/macrophage cell line in the red pulp and marginal zone of the mouse spleen, where ephrin-A1 is prominently expressed in the vasculature Histochem. Cell Biol. Sep 24 2016 [PMID: 27665280] (Mouse) Mouse
Yin SY, Jian FY, Chen YH et al. Induction of IL-25 secretion from tumour-associated fibroblasts suppresses mammary tumour metastasis. Nat Commun. 2016 Apr 19 [PMID: 27089063] (Flow, Mouse)

Details:
This citation used the Alexa Fluor 647 version of this antibody.
Flow Mouse
McGee HM, Schmidt BA, Booth CJ et al. IL-22 Promotes Fibroblast-Mediated Wound Repair in the Skin J Invest Dermatol 2012 Dec 6 [PMID: 23223145] (FLOW, IHC, ICC/IF, Mouse) FLOW, IHC, ICC/IF Mouse
Mandl JN, Liou R, Klauschen F et al. Quantification of lymph node transit times reveals differences in antigen surveillance strategies of naive CD4+ and CD8+ T cells Proc Natl Acad Sci 2012 Oct 15 [PMID: 23071319] (IHC, ICC/IF, Mouse) IHC, ICC/IF Mouse
Wang X, Cho B, Suzuki K et al. Follicular dendritic cells help establish follicle identity and promote B cell retention in germinal centers. The Journal of Experimental Medicine. 2011 Oct 31. [PMID: 22042977]
Treiber N, Maity P, Singh K et al. Accelerated aging phenotype in mice with conditional deficiency for mitochondrial superoxide dismutase in the connective tissue. Aging Cell. 2010 Nov 25. doi: 10.1111/j.1474-9726.2010.00658.x. 2010 Nov 25. [PMID: 21108731]

Review for Fibroblast Antibody (NB100-64932) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: IHC-Fr.

Reviews using NB100-64932:
Filter by Applications
IHC-Fr
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Fibroblast NB100-64932
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reviewed by:
Anonymous
IHC-Fr Mouse 06/29/2017
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedPFA Fixed Frosen Section Mouse Lymphnode
SpeciesMouse

Comments

CommentsWD [1:500]; secondary ab RTU Polymer-HRP

Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for Fibroblast Antibody (NB100-64932). (Showing 1 - 1 of 1 FAQs).

  1. I am currently in search of an antibody for staining of human dermal fibroblasts. Can you recommend me an antibody for this? Is there an antibody that specifically stains only fibroblasts and not mesenchymal stem cells?
    • We sell a number of antibodies which are suitable for staining fibroblasts. You can see these at the following links: NB100-64932, NB110-15846 and NB100-1845. NB100-64932 recognizes ER-TR7, an antigen that is located in the cytoplasm of reticular fibroblasts and is a component of the extracellular matrix of lymphoid and non-lymphoid organs. The antigen recognized by clone ER-TR7 has not been identified but studies suggest that it is likely to be distinct from laminin, fibronectin, collagen types I-IV, heparin sulphate proteoglycan, entactin and nidogen. NB110-15846 is specific to beta subunit of prolyl 4-hydroxylase. It reacts with fibroblasts and myoepithelial cells, and does not stain lymphocytes, monocytes, dendritic cells or granulocytes. NB100-1845 recognizes an epitope on primary cultured human fibroblasts, fibroblast cell lines, tissue macrophages and 95% of peripheral blood monocytes. It does not react with vascular smooth muscle cells. Unfortunately, we do not have any data regarding the staining of mesenchymal stem cells with any of these antibodies in our QC lab.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-64932AF405
Alexa Fluor 488 Labeled NB100-64932AF488
Alexa Fluor 647 Labeled NB100-64932AF647
Alexa Fluor 700 Labeled NB100-64932AF700
Allophycocyanin Labeled NB100-64932APC
DyLight 350 Labeled NB100-64932UV
DyLight 405 Labeled NB100-64932V
DyLight 488 Labeled NB100-64932G
DyLight 680 Labeled NB100-64932FR
DyLight 755 Labeled NB100-64932IR
PE Labeled NB100-64932PE
PerCP Labeled NB100-64932PCP

Additional Fibroblast Products

Fibroblast NB100-64932

Research Areas for Fibroblast Antibody (NB100-64932)

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Blogs on Fibroblast

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Recent Reviews

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Anonymous
06/29/2017
Application: IHC-Fr
Species: Mouse