Fibroblast Antibody (ER-TR7) - BSA Free

Immunohistochemistry: Fibroblast Antibody (ER-TR7) [NB100-64932] - (Ai-Aii) Representative ER-TR7 stained sections from xenograft tumors harvested at day 7. (Ai) Untreated, (Aii) Pt. Image collected and cropped by CiteAb from the following publication (onlinelibrary.wiley.com/doi/full/10.1002/jbm.b.34254), licensed under a CC-BY license.

Immunohistochemistry-Frozen: Fibroblast Antibody (ER-TR7) [NB100-64932] - Fibroblast Antibody (ER-TR7) IHC on Mouse lymph node, frozen section, 20x. Primary antibody diluted 1:500. This image was submitted via customer Review.

• Reactivity: Mu
• Applications: Flow, ICC/IF, IHC
• Clone: ER-TR7
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Fibroblast Antibody (ER-TR7) - BSA Free Summary

• Immunogen: Isolated C3H thymic stromal cells
• Marker: Fibroblast Marker
• Specificity: NB100-64932 recognizes ER-TR7, an antigen that is located in the cytoplasm of reticular fibroblasts and is a component of the extracellular matrix of lymphoid and non-lymphoid organs. The antigen recognized by clone ER-TR7 has not been identified but studies suggest that it is likely to be distinct from laminin, fibronectin, collagen types I-IV, heparin sulphate proteoglycan, entactin and nidogen. Clone ER-TR7 has been used to stain the microanatomy of various organs and also stains subendothelial deposits in atherosclerotic plaques.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:300-1:500. Use reported in scientific literature (PMID 22042977)
  • Immunohistochemistry-Frozen 1:500
  • Immunohistochemistry-Paraffin reported in scientific literature (PMID 35794106)
• Application Notes: Membrane permeabilisation is required for Flow Cytometry.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Fibroblast Antibody (ER-TR7) - BSA Free

• reticular fibroblast

Background

Connective tissue consists of a ground of glycosaminoglycans, proteoglycans and glycoproteins through which various fibres run. These fibres can be collagenous, elastic or reticular. Reticular fibres are composed from the family of collagen proteins and give tensile strength. These fibres are made by reticular fibroblasts. This monoclonal antibody [ER-TR7] detects an antigen present in, and produced by, reticular fibroblasts. The antibody is useful to stain the microanatomy of various organs, in particular the connective tissue framework in lymphoid organs. The antibody also stains subendothelial deposits in the plaque area of atherosclerotic plaques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fibroblast Antibody (NB100-64932)(28)

Publications using NB100-64932

• Taylor X, Noristani HN, Fitzgerald GJ et al. Amyloid-beta (A beta ) immunotherapy induced microhemorrhages are linked to vascular inflammation and cerebrovascular damage in a mouse model of Alzheimer’s disease Molecular Neurodegeneration 2024-10-21 [PMID: 39434125]
• Watson SS, Zomer A, Fournier N, Lourenco J et Al. Fibrotic response to anti-CSF-1R therapy potentiates glioblastoma recurrence Cancer Cell 2024-09-10 [PMID: 39255775]
• Bejarano L, Kauzlaric A, Lamprou E, Lourenco J et Al. Interrogation of endothelial and mural cells in brain metastasis reveals key immune-regulatory mechanisms Cancer Cell 2024-01-19 [PMID: 38242126]
• Plá V, Bitsika S, Giannetto M et al. Structural characterization of SLYM - a 4thmeningeal membrane bioRxiv 2023-10-24 [PMID: 38098084] (IHC-P, Mouse)
• Ugur M, Labios RJ, Fenton C et al. Lymph node medulla regulates the spatiotemporal unfolding of resident dendritic cell networks Immunity 2023-07-07 [PMID: 37463581] (ICC/IF)
  
  Details:
  Alexa Fluor 700 conjugation used
• Ontsouka E, Schroeder M, Ok L et al. The Placenta-A New Source of Bile Acids during Healthy Pregnancy? First Results of a Gene Expression Study in Humans and Mice International journal of molecular sciences 2023-05-30 [PMID: 37298459] (Flow Cytometry, Human)
• Mestre H, Verma N, Greene TD et al. Periarteriolar spaces modulate cerebrospinal fluid transport into brain and demonstrate altered morphology in aging and Alzheimer's disease Nature communications 2022-07-06 [PMID: 35794106] (IHC-P, IF/IHC, Mouse)
• McCorkell KA, Jayachandran N, Cully MD Et al. Lymph node formation and B cell homeostasis require IKK-alpha in distinct endothelial cell-derived compartments Proceedings of the National Academy of Sciences of the United States of America 2021-11-30 [PMID: 34810256]
• Lu W, Meng Z, Hernandez R, Zhou C. Fibroblast-specific IKK-? deficiency ameliorates angiotensin II-induced adverse cardiac remodeling in mice JCI Insight 2021-09-22 [PMID: 34324438] (Immunohistochemistry, Mouse)
• Ishigaki K, Kumano K, Fujita K, Ueno H Cellular basis of omentum activation and expansion revealed by single-cell RNA sequencing using a parabiosis model Sci Rep 2021-07-07 [PMID: 34230565]
• Alam J, Ogawa K Expression and localisation of ephrin-B1 and EphB4 in steroidogenic cells in the naturally cycling mouse ovary Reproductive biology 2021-05-12 [PMID: 33991765]
• Dorrier CE, Aran D, Haenelt EA et al. CNS fibroblasts form a fibrotic scar in response to immune cell infiltration Nature neuroscience 2021-02-01 [PMID: 33526922]
• Bianco R, Di Gregoli K, Caputo M et al. A Protocol for a Novel Human Ex Vivo Model of Aneurysm STAR Protocols 2020-12-30 [PMID: 33377004] (Immunohistochemistry, Mouse)
• Kim SH, Singh R, Han C et al Chronic activation of 4-1BB signaling induces granuloma development in tumor-draining lymph nodes that is detrimental to subsequent CD8(+) T cell responses Cell Mol Immunol 2020-09-02 [PMID: 32868911] (IHC-Fr, Mouse)
  
  Details:
  Citation using the DyLight 488 version of this antibody.
• Gofur M, Alam J, Ogawa K Expression and localisation of ephrin-B1, EphB2, and EphB4 in the mouse testis during postnatal development Reproductive Biology 2020-07-01 [PMID: 32620380] (ICC/IF, Mouse)
• Kitamura T, Kato Y et al. Mammary Tumor Cells with High Metastatic Potential Are Hypersensitive to Macrophage-Derived HGF. Cancer Immunol Res 2019-01-12 [PMID: 31615815] (ICC/IF, Mouse)
• Gray ME, Meehan J, Blair EO et al. Biocompatibility of common implantable sensor materials in a tumor xenograft model. J. Biomed. Mater. Res. Part B Appl. Biomater. 2018-10-27 [PMID: 30367816] (IF/IHC, Mouse)
• Gofur MR, Ogawa K. Compartments with predominant ephrin-B1 and EphB2/B4 expression are present alternately along the excurrent duct system in the adult mouse testis and epididymis. Andrology 2018-07-25 [PMID: 30043555] (Mouse)
• Konda N, Saeki N, Nishino S, Ogawa K. Truncated EphA2 likely potentiates cell adhesion via integrins as well as infiltration and/or lodgment of a monocyte/macrophage cell line in the red pulp and marginal zone of the mouse spleen, where ephrin-A1 is prominently expressed in the vasculature Histochem. Cell Biol. 2016-09-24 [PMID: 27665280] (Mouse)
• Yin SY, Jian FY, Chen YH et al. Induction of IL-25 secretion from tumour-associated fibroblasts suppresses mammary tumour metastasis. Nat Commun. 2016-04-19 [PMID: 27089063] (FLOW, Mouse)
  
  Details:
  This citation used the Alexa Fluor 647 version of this antibody.
• McGee HM, Schmidt BA, Booth CJ et al. IL-22 Promotes Fibroblast-Mediated Wound Repair in the Skin J Invest Dermatol 2012-12-06 [PMID: 23223145] (IF/IHC, FLOW, ICC/IF, Mouse)
• An S, Tiruthani K, Wang Y et al. Locally Trapping the C-C Chemokine Receptor Type 7 by Gene Delivery Nanoparticle Inhibits Lymphatic Metastasis Prior to Tumor Resection Small 2019-01-28 [PMID: 23071319] (IF/IHC, ICC/IF, Mouse)
• Wang X, Cho B, Suzuki K et al. Follicular dendritic cells help establish follicle identity and promote B cell retention in germinal centers. The Journal of Experimental Medicine. 2011-10-31 [PMID: 22042977]
• Joe A W, Yi L et al. Muscle injury activates resident fibro/adipogenic progenitors that facilitate myogenesis. Nat Cell Biol 2010-01-02 [PMID: 20081841] (ICC/IF, Mouse)
• Mello Coelho Valeria De, Bunbury Allyson, Rangel Leticia B et al. Fat-storing multilocular cells expressing CCR5 increase in the thymus with advancing age: potential role for CCR5 ligands on the differentiation and migration of preadipocytes. Int J Med Sci 2009-01-01 [PMID: 20046229] (ICC/IF, Mouse)
• Kuhlmann M T, Kirchhof P et al. G-CSF/SCF reduces inducible arrhythmias in the infarcted heart potentially via increased connexin43 expression and arteriogenesis. J Exp Med 2006-01-23 [PMID: 16401694] (IF/IHC, Mouse)
• Quintana J, Sinton M, Chandrasegaran P et al. The murine meninges acquire lymphoid tissue properties and harbour autoreactive B cells during chronicTrypanosoma bruceiinfection bioRxiv 2023-04-30 (Immunohistochemistry-Paraffin, Mouse)
  
  Details:
  IHC-P 1:100
• Treiber N, Maity P, Singh K et al. Accelerated aging phenotype in mice with conditional deficiency for mitochondrial superoxide dismutase in the connective tissue. Aging Cell. 2010 Nov 25. doi: 10.1111/j.1474-9726.2010.00658.x. 2010-11-25 [PMID: 21108731]

Review for Fibroblast Antibody (NB100-64932) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IHC Mouse 06/29/2017

Summary

• Application: Immunohistochemistry
• Sample Tested: PFA Fixed Frosen Section Mouse Lymphnode
• Species: Mouse

Comments

• Comments: WD [1:500]; secondary ab RTU Polymer-HRP

FAQs for Fibroblast Antibody (NB100-64932). (Showing 1 - 1 of 1 FAQs).

1. I am currently in search of an antibody for staining of human dermal fibroblasts. Can you recommend me an antibody for this? Is there an antibody that specifically stains only fibroblasts and not mesenchymal stem cells?
   • We sell a number of antibodies which are suitable for staining fibroblasts. You can see these at the following links: NB100-64932, NB110-15846 and NB100-1845. NB100-64932 recognizes ER-TR7, an antigen that is located in the cytoplasm of reticular fibroblasts and is a component of the extracellular matrix of lymphoid and non-lymphoid organs. The antigen recognized by clone ER-TR7 has not been identified but studies suggest that it is likely to be distinct from laminin, fibronectin, collagen types I-IV, heparin sulphate proteoglycan, entactin and nidogen. NB110-15846 is specific to beta subunit of prolyl 4-hydroxylase. It reacts with fibroblasts and myoepithelial cells, and does not stain lymphocytes, monocytes, dendritic cells or granulocytes. NB100-1845 recognizes an epitope on primary cultured human fibroblasts, fibroblast cell lines, tissue macrophages and 95% of peripheral blood monocytes. It does not react with vascular smooth muscle cells. Unfortunately, we do not have any data regarding the staining of mesenchymal stem cells with any of these antibodies in our QC lab.