Feline Leukaemia Virus p15E Antibody (PF6J-2A1) - BSA Free

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Product Details

Summary
Reactivity V-ViSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clone
PF6J-2A1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Feline Leukaemia Virus p15E Antibody (PF6J-2A1) - BSA Free Summary

Immunogen
Made to Viral FELINE LEUKAEMIA VIRUS p15E
Specificity
NB100-65808 recognizes an epitope within the amino-terminal domain of both the 20kDa full-length (including intracellular sequence), and the 15kDa truncated forms, of feline leukaemia virus (FeLV) p15e transmembrane envelope protein. Persistent infection of cats with the oncoretrovirus FeLV can result in the non-neoplastic condition known as FeLV-associated enteritis (FAE). Studies have shown strong expression of the major FeLV proteins p15e and gp70 in intestinal crypt epithelial cells of cats with FAE, compared to FeLV-positive cats without intestinal alterations, thereby suggesting a pathogenic role for these envelope proteins/precursors in FAE.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:2000
Application Notes
In WB detects band/s of approximately 15kDa and 20kDa under reducing conditions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Feline Leukaemia Virus p15E Antibody (PF6J-2A1) - BSA Free

  • Env polyprotein
  • Feline Leukemia Virus glycoprotein 70
  • Glycoprotein gp70 [Precursor]
  • GP70 envelope protein
  • gp70 protein (SU)
  • p15E protein (TM)
  • p15E protein

Background

FeLV is a retrovirus (family Oncovirinae) that can cause severe immunosuppression, severe non regenerative anemia, and a variety of neoplasms. The 3 main subtypes (FeLVA, FeLVB and FeLVC) may act independently or together to cause a variety of diseases. The major envelope glycoprotein (gp70) is used to bind to target cells and varies with the FeLV subtype.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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