ESGP Antibody [Unconjugated]

Western blot shows lysates of D3 mouse embryonic stem cell line. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Mouse ESGP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4580) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ESGP at approximately 9 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

ESGP was detected in perfusion fixed frozen sections of mouse testis using Sheep Anti-Mouse ESGP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4580) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Northern­Lights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to late spermatids. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

ESGP was detected in immersion fixed D3 mouse embryonic stem cell line using Sheep Anti-Mouse ESGP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4580) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell secretion. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

• Reactivity: Mu
• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

ESGP Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse ESGP
  Leu24-Lys84
  Accession # Q2Q5T5
• Specificity: Detects mouse ESGP in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: Gm7325
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ESGP Antibody [Unconjugated]

• EG653016
• EG653016;Gm7325;m;minion;Mymx;myomer;myomerger;Protein myomixer
• ESGP

Background

ESGP (ES cell and germ cell-specific protein) is a presumably secreted polypeptide that belongs to no known protein family. It is expressed specifically in pluripotential cell types, and does not appear to be involved in differentiation. Mouse ESGP precursor is 84 amino acids (aa) in length. It contains a 25 aa signal sequence with a 59 aa mature segment. There are no N-linked glycosylation sites. One potential 108 aa variant shows an alternate start site 24 aa upstream of the standard start site.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ESGP Antibody (AF4580)(13)

Publications using AF4580

• Kurosaka, M;Hung, YL;Machida, S;Kohda, K; IL-4 Signaling Promotes Myoblast Differentiation and Fusion by Enhancing the Expression of MyoD, Myogenin, and Myomerger Cells 2023-04-29 [PMID: 37174683] (Western Blot, Mouse)
• DG Gamage, K Melikov, P Munoz-Tell, TJ Wherley, LC Focke, E Leikina, E Huffman, J Diao, DJ Kojetin, V Prasad, LV Chernomord, DP Millay Phosphatidylserine orchestrates Myomerger membrane insertions to drive myoblast fusion Proceedings of the National Academy of Sciences of the United States of America, 2022-09-12;119(38):e2202490119. 2022-09-12 [PMID: 36095199] (ICC, Mouse)
• JH Yang, MW Chang, D Tsitsipati, X Yang, JL Martindale, R Munk, A Cheng, E Izydore, PR Pandey, Y Piao, K Mazan-Mamc, S De, K Abdelmohse, M Gorospe LncRNA OIP5-AS1-directed miR-7 degradation promotes MYMX production during human myogenesis Nucleic Acids Research, 2022-07-08;0(0):. 2022-07-08 [PMID: 35736212] (Western Blot, Human)
• M Zocchi, L Locatelli, GV Zuccotti, A Mazur, D Béchet, JA Maier, S Castiglion Magnesium Homeostasis in Myogenic Differentiation-A Focus on the Regulation of TRPM7, MagT1 and SLC41A1 Transporters International Journal of Molecular Sciences, 2022-01-31;23(3):. 2022-01-31 [PMID: 35163580] (Western Blot, Mouse)
• Hammers DW, Hart CC, Matheny MK et al. Filopodia powered by class x myosin promote fusion of mammalian myoblasts eLife 9/14/2021 [PMID: 34519272]
• Hammers DW, Hart CC, Matheny MK et al. Filopodia powered by class x myosin promote fusion of mammalian myoblasts eLife 2021-09-14 [PMID: 34519272]
• Nikki M. McCormack, Eric Villalón, Coralie Viollet, Anthony R. Soltis, Clifton L. Dalgard, Christian L. Lorson et al. Survival motor neuron deficiency slows myoblast fusion through reduced myomaker and myomixer expression Journal of Cachexia, Sarcopenia and Muscle 8/1/2021 [PMID: 34115448]
• Lilya Lehka, Małgorzata Topolewska, Dominika Wojton, Olena Karatsai, Paloma Alvarez-Suarez, Paweł Pomorski et al. Formation of Aberrant Myotubes by Myoblasts Lacking Myosin VI Is Associated with Alterations in the Cytoskeleton Organization, Myoblast Adhesion and Fusion Cells 7/11/2020 [PMID: 32664530]
• Domiziana Costamagna, Robin Duelen, Fabio Penna, Detlef Neumann, Paola Costelli, Maurilio Sampaolesi Interleukin‐4 administration improves muscle function, adult myogenesis, and lifespan of colon carcinoma‐bearing mice Journal of Cachexia, Sarcopenia and Muscle 6/1/2020 [PMID: 32103619]
• Evgenia Leikina, Dilani G. Gamage, Vikram Prasad, Joanna Goykhberg, Michael Crowe, Jiajie Diao et al. Myomaker and Myomerger Work Independently to Control Distinct Steps of Membrane Remodeling during Myoblast Fusion Developmental Cell 9/6/2018 [PMID: 30197239]
• P Bi, JR McAnally, JM Shelton, E Sánchez-Or, R Bassel-Dub, EN Olson Fusogenic micropeptide Myomixer is essential for satellite cell fusion and muscle regeneration Proc. Natl. Acad. Sci. U.S.A., 2018-03-26;0(0):. 2018-03-26 [PMID: 29581287] (Western Blot, Mouse)
• ME Quinn, Q Goh, M Kurosaka, DG Gamage, MJ Petrany, V Prasad, DP Millay Myomerger induces fusion of non-fusogenic cells and is required for skeletal muscle development Nat Commun, 2017-06-01;8(0):15665. 2017-06-01 [PMID: 28569755] (Western Blot, Mouse)
• Q Zhang, AA Vashisht, J O'Rourke, SY Corbel, R Moran, A Romero, L Miraglia, J Zhang, E Durrant, C Schmedt, SC Sampath, SC Sampath The microprotein Minion controls cell fusion and muscle formation Nat Commun, 2017-06-01;8(0):15664. 2017-06-01 [PMID: 28569745] (ICC, IHC, Mouse)

Review for ESGP Antibody (AF4580) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer KO Mouse 10/16/2020

Summary

• Application: Knockout Validated
• Sample Tested: Muscle tissue
• Species: Mouse
• Lot: CAGA0220031

Comments

• Comments: Specificity validated by KO samples ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***

Bioinformatics

• Gene Symbol: Gm7325
• Uniprot:
  • Q2Q5T5()