ER-HR3 Antibody (ER-HR3) [Biotin]

Product Discontinued

ER-HR3 Antibody (ER-HR3) [Biotin] Summary

• Description: This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
• Immunogen: Adherent F1 (CBAxBL) bone marrow stormal cells.
• Specificity: NB100-64931 recognizes the murine antigen ER-HR3. ER-HR3 is a cell surface antigen which is expressed by Langerhans cells in epithelium, a subset of mature macrophages and dendritic cells that are located predominantly in haematopoietic and lymphoid organs. Initial studies of ER-HR3 demonstrated expression on peripheral blood monocytes but recent studies suggest that expression is actually very low. During foetal development, ER-HR3 positive cells are localised to haemapoietic islands and are often associated with erythroid progenitor cells. The functions of the ER-HR3 antigen have not been established but reports suggest that the antigen may be involved in adult erythropoiesis and in the regulation of the immune response. Clone ER-HR3 does not inhibit T cell proliferation in antigen-specific T-cell proliferation studies. Clone ER-HR3 recognizes two proteins of approximately 69kD and 55kD under non-reducing conditions.
• Isotype: IgG2c
• Clonality: Monoclonal
• Host: Rat
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for ER-HR3 Antibody (ER-HR3) [Biotin]

• Hematopoiesis related macrophage

Background

Macrophage are any of the many forms of mononuclear phagocytes found in tissues. Mononuclear phagocytes arise from hematopoietic stem cells in the bone marrow. After passing through the monoblast and promonocyte states of the monocyte stage, they enter the blood, circulating for about 40 hours. They then enter tissues and increase in size, phagocytic activity, and lysosomal enzyme content and become macrophages. The morphology of macrophages varies among different tissues and between normal and pathologic states, and not all macrophages can be identified by morphology alone. However, most macrophages are large cells with a round or indented nucleus, a well developed Golgi apparatus, abundant endocytotic vacuoles, lysosomes, and phagolysosomes, and a plasma membrane covered with ruffles or microvilli. Among the functions of macrophages are nonspecific phagocytosis and pinocytosis, specific phagocytosis of opsonized microorganisms mediated by Fc receptors and complement receptors, killing of ingested microorganisms, digestion and presentation of antigens to T and B lymphocytes, and secretion of a large number of diverse products, including many enzymes (lysozyme, collagenases, elastase, acid hydrolases), several complement components and coagulation factors, some prostaglandins and leukotrienes, and several regulatory molecules (interferon, interleukin 1). Among the cells now recognized as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans type and foreign body type multinucleated giant cells (in inflamed tissues).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ER-HR3 Antibody (NB100-64931B)(1)

Publication using NB100-64931B

• Kaur S, Raggatt LJ, Millard SM et al. Self-repopulating recipient bone marrow resident macrophages promote long-term hematopoietic stem cell engraftment. Blood 2018-06-26 [PMID: 29945953] (IF/IHC, Mouse)