![ELISA: Enterovirus D68 VP1 Antibody (GT11610) [NBP3-13557] - Sandwich ELISA detection of recombinant full-length VP1 protein(s) derived from different strains of Enterovirus (ie., D68; 71) using antibodies as below. Capture: Enterovirus D68 VP1 antibody [GT11610] (NBP3-13557) (5 ug/mL) Detection: Enterovirus D68 VP1 antibody [HL1997] (1 ug/mL)](http://images.novusbio.com/fullsize/nbp3-13557_mouse-enterovirus-d68-vp1-mab-gt11610-910202420104491.jpg "ELISA: Enterovirus D68 VP1 Antibody (GT11610) [NBP3-13557] - Sandwich ELISA detection of recombinant full-length VP1 protein(s) derived from different strains of Enterovirus (ie., D68; 71) using antibodies as below. Capture: Enterovirus D68 VP1 antibody [GT11610] (NBP3-13557) (5 ug/mL) Detection: Enterovirus D68 VP1 antibody [HL1997] (1 ug/mL)")
| Reactivity | V-ViSpecies Glossary |
| Applications | WB |
| Clone | GT11610 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Description | Centrifuge briefly prior to opening. |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of Enterovirus D68 VP1 protein. The exact sequence is proprietary. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS, 20% Glycerol |
| Preservative | No Preservative |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus D68 VP1 antibody [GT11610] (NBP3-13557) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13557_mouse-enterovirus-d68-vp1-mab-gt11610-western-blot-261120251821331.jpg "Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus D68 VP1 antibody [GT11610] (NBP3-13557) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.")
![Non-infected (-) and infected (+) RD whole cell extracts (5 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus D68 VP1 antibody [GT11610] (NBP3-13557) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13557_mouse-enterovirus-d68-vp1-mab-gt11610-western-blot-2611202518363311.jpg "Non-infected (-) and infected (+) RD whole cell extracts (5 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus D68 VP1 antibody [GT11610] (NBP3-13557) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.")
![Enterovirus D68 VP1 antibody [GT11610] detects Enterovirus D68 VP1 protein by immunohistochemical analysis.Sample: Paraffin-embedded mock and Enterovirus D68 VP1 transfected 293T cell.Green: Enterovirus D68 VP1 stained by Enterovirus D68 VP1 antibody [GT11610] (NBP3-13557) diluted at 1:4000.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13557_mouse-enterovirus-d68-vp1-mab-gt11610-immunohistochemistry-paraffin-2611202518422917.jpg "Enterovirus D68 VP1 antibody [GT11610] detects Enterovirus D68 VP1 protein by immunohistochemical analysis.Sample: Paraffin-embedded mock and Enterovirus D68 VP1 transfected 293T cell.Green: Enterovirus D68 VP1 stained by Enterovirus D68 VP1 antibody [GT11610] (NBP3-13557) diluted at 1:4000.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Enterovirus D68 VP1 antibody [GT11610] detects Enterovirus D68 VP1 protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Enterovirus D68 VP1 stained by Enterovirus D68 VP1 antibody [GT11610] (NBP3-13557) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-13557_mouse-enterovirus-d68-vp1-mab-gt11610-immunocytochemistry-immunofluorescence-261120251842540.jpg "Enterovirus D68 VP1 antibody [GT11610] detects Enterovirus D68 VP1 protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Enterovirus D68 VP1 stained by Enterovirus D68 VP1 antibody [GT11610] (NBP3-13557) diluted at 1:500.Blue: Fluoroshield with DAPI .")
![Indirect ELISA analysis was performed by coating the plate with recombinant full-length enterovirus D68 VP1 (4000-62.5 ng/mL). Coated protein was probed with Enterovirus D68 VP1 antibody [GT11610] (NBP3-13557) (1 ug/mL). Goat anti-mouse IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-13557_mouse-enterovirus-d68-vp1-mab-gt11610-elisa-261120251971810.jpg "Indirect ELISA analysis was performed by coating the plate with recombinant full-length enterovirus D68 VP1 (4000-62.5 ng/mL). Coated protein was probed with Enterovirus D68 VP1 antibody [GT11610] (NBP3-13557) (1 ug/mL). Goat anti-mouse IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.")
![Immunohistochemistry-Paraffin: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] [NB7539] - FFPE serial sections of human stomach carcinoma. Primary Antibody: Mouse anti-p53 (Clone DO-1) used at a dilution of 1:100. Secondary Antibody: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] Conjugated used at a dilution of 1:200 (5ug/ml). Detection: DAB](https://images.novusbio.com/images/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-HRP-Immunohistochemistry-Paraffin-NB7539-img0014.jpg "Immunohistochemistry-Paraffin: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] [NB7539] - FFPE serial sections of human stomach carcinoma. Primary Antibody: Mouse anti-p53 (Clone DO-1) used at a dilution of 1:100. Secondary Antibody: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] Conjugated used at a dilution of 1:200 (5ug/ml). Detection: DAB")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")