![ELISA: Enterovirus 71 VP1 Antibody (GT185) [NBP3-13536] - Indirect ELISA analysis was performed by coating the plate with recombinant full-length enterovirus 71 VP1 (4000-62.5 ng/mL). Coated protein was probed with Enterovirus 71 VP1 antibody [GT185] (NBP3-13536) (1 ug/mL). Goat anti-mouse IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-13536_mouse-enterovirus-71-vp1-mab-gt185-91020242011474.jpg "ELISA: Enterovirus 71 VP1 Antibody (GT185) [NBP3-13536] - Indirect ELISA analysis was performed by coating the plate with recombinant full-length enterovirus 71 VP1 (4000-62.5 ng/mL). Coated protein was probed with Enterovirus 71 VP1 antibody [GT185] (NBP3-13536) (1 ug/mL). Goat anti-mouse IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.")
| Reactivity | V-ViSpecies Glossary |
| Applications | WB |
| Clone | GT185 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Description | Centrifuge briefly prior to opening. |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of Enterovirus 71 VP1 (EV71 virus (strain USA/BrCr/1970)). The exact sequence is proprietary. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
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![Enterovirus 71 VP1 antibody [GT185] detects Enterovirus 71 VP1 protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Enterovirus 71 VP1 stained by Enterovirus 71 VP1 antibody [GT185] (NBP3-13536) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-13536_mouse-enterovirus-71-vp1-mab-gt185-immunocytochemistry-immunofluorescence-26112025196222.jpg "Enterovirus 71 VP1 antibody [GT185] detects Enterovirus 71 VP1 protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Enterovirus 71 VP1 stained by Enterovirus 71 VP1 antibody [GT185] (NBP3-13536) diluted at 1:500.Blue: Fluoroshield with DAPI .")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")