ELFN1 Antibody (965705) [Alexa Fluor™ Plus 647]

• Reactivity: Hu
• Clone: 965705
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 647

ELFN1 Antibody (965705) [Alexa Fluor™ Plus 647] Summary

• Specificity: Detects human ELFN1 in direct ELISAs.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ELFN1 Antibody (965705) [Alexa Fluor™ Plus 647]

• ELFN1
• PPP1R28
• USP17L7

Background

ELFN1 (Extracellular leucine-rich repeat and fibronectin type-III domain-containing protein 1), also known as Protein phosphatase 1 regulatory subunit 28, is a member of the extracellular Leucine-Rich Repeat superfamily (1). Expressed mainly in the nervous system, ELFN1 is a transmembrane protein that inhibits the activity of protein phosphatase 1 (PP1) complexes (2). Mature human ELFN1 consists of a 391 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment and a 389 aa cytoplasmic tail. The ECD includes one fibronectin type-III domain, six leucine-rich repeats (LRR) and one LRR C-terminal (LRRCT) domain. Human ELFN1 shares 90% aa sequence identity with mouse and rat ELFN1. The cytoplasmic tail contains many tyrosine but no other detectable motifs. ELFN1 is strongly expressed in globus pallidus and interneurons in cortex and hippocampus in both developing and adult brains (1). It is also expressed in endocrine and reproductive tissues (1). Given the functions and discrete patterns of many known LRR family proteins, it has been proposed that ELFN1 could serve as a neuronal adhesion molecule and play an integral role in synapse formation and differentiation via the coordination of both pre- and postsynaptic machineries, thereby involved in neurite outgrowth, axon guidance, fasciculation, and synapse formation (3). Recent studies showed that ELFN1 physically anchor metabotropic glutamate receptor 6 (mGluR6) and mGluR7 across retinal and hippocampal synapses (3-4), and can be recruited selectively to all group III mGluRs (mGluR4, mGluR6, mGluR7, and mGluR8) to allosterically modulate these receptors (5).

1. Dolan J. et al. (2007) BMC Genomics 8:320.
2. Hendrickx, A. et al. (2009) Chem. Biol. 16:365.
3. Williams, M.E. et al. (2010) Neuron 68:9.
4. De Wit, J. and Ghosh A. (2016) Nat Rev Neurosci 17:22.
5. Dunn H. A. et al. (2018) PNAS 115:5022.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.