![Flow (Intracellular): EBV Latent Membrane Protein 1 Antibody (LMPO24) [NBP2-50383] - Intracellular staining of LMP1 using LMPO24 antibody. NK and T cell lines were fixed and permeabilised with an EBiosciences kit (Cat 88-8824-00) then stained with LMPO24 monoclonal antibody, followed by detection with Alexa Fluor 488 conjugated anti-mouse IgG antibody and analysis by flow cytometry. The histograms show negative control fluorescence staining (black lines) and LMPO24 staining (red lines). MEC04, SNK1, SNK6 and SNT8 are EBV positive tumour cell lines derived from patients with nasal NK/T cell lymphomas. MEC04, SNK1 and SNK6 are NK cell lines, while SNT8 is a T cell line. MEC04 lacks LMP1 expression, SNK1 expresses weak to moderate levels of LMP1, whilst SNK6 and SNT8 express high levels of LMP1.](http://images.novusbio.com/fullsize/EBV-Latent-Membrane-Protein-1-Antibody-LMPO24-Flow-Intracellular-NBP2-50383-img0001.jpg "Flow (Intracellular): EBV Latent Membrane Protein 1 Antibody (LMPO24) [NBP2-50383] - Intracellular staining of LMP1 using LMPO24 antibody. NK and T cell lines were fixed and permeabilised with an EBiosciences kit (Cat 88-8824-00) then stained with LMPO24 monoclonal antibody, followed by detection with Alexa Fluor 488 conjugated anti-mouse IgG antibody and analysis by flow cytometry. The histograms show negative control fluorescence staining (black lines) and LMPO24 staining (red lines). MEC04, SNK1, SNK6 and SNT8 are EBV positive tumour cell lines derived from patients with nasal NK/T cell lymphomas. MEC04, SNK1 and SNK6 are NK cell lines, while SNT8 is a T cell line. MEC04 lacks LMP1 expression, SNK1 expresses weak to moderate levels of LMP1, whilst SNK6 and SNT8 express high levels of LMP1.")
| Reactivity | V-ViSpecies Glossary |
| Applications | Flow, IHC, CyTOF-ready |
| Clone | LMPO24 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Description | Novus Biologicals Mouse EBV Latent Membrane Protein 1 Antibody (LMPO24) - BSA Free (NBP2-50383) is a monoclonal antibody validated for use in IHC and Flow. Anti-EBV Latent Membrane Protein 1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Purified plasma membranes from EBV Latent Membrane Protein 1 expressing insect cells infected with a recombinant EBV Latent Membrane Protein 1 baculovirus |
| Specificity | Niedobitek et al., 1995 (PMID: 7605996): "The LMPO24 clone demonstrates strong and specific reactivity in immunohistochemical staining of paraffin-embedded tissues." |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Positive control(s): EBV transformed lymphoblastoid cell lines. This antibody is CyTOF-ready. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
| Publication using NBP2-50383 | Applications | Species |
|---|---|---|
| Chakravorty S, Yan B, Wang C et al. Integrated pan-cancer map of EBV-associated neoplasms reveals functional host-virus interactions Cancer Res. 2019-09-03 [PMID: 31481499] (FLOW, Virus) | FLOW | Virus |
Secondary Antibodies |
Isotype Controls |
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")