EBOV GP Antibody (993408) [Unconjugated]

Western blot shows Recombinant EBOV GP. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Viral EBOV GP Monoclonal Antibody (Catalog # MAB9016) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for EBOV GP at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: V
• Applications: WB
• Clone: 993408
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

EBOV GP Antibody (993408) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant Viral EBOV GP
  Ile33-Arg501
  Accession # Q05320
• Specificity: Detects EBOV GP in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EBOV GP Antibody (993408) [Unconjugated]

• bovGP
• EBOV GP
• second secreted glycoprotein
• small secreted glycoprotein
• Spike glycoprotein

Background

The GP glycoprotein encoded by the genome of Ebola family viruses is a critical molecule for the pathogenicity of Ebolavirus hemorrhagic viruses (1, 2). It is processed into distinct forms for virus capsule or cell surface presentation or release from virus infected cells. The GP precursor protein is cleaved by furin at a multibasic site to yield a 140 kDa N-terminal fragment (GP1) and a 26 kDa C-terminal fragment (GP2) which remain disulfide linked (3). GP1 is entirely extracellular while GP2 is a transmembrane protein (4). Heterodimers of GP1-GP2 can further associate into trimers (5). GP expressed on virus infected cells can be shed by TACE mediated cleavage, liberating a disulfide linked complex of soluble GP1 and truncated GP2 (4-6). GP binds to multiple C-type lectins on target cell surfaces, including CLEC10A/MGL, DC-SIGN, and DC-SIGNR (7-9). Following internalization, GP1 is cleaved by Cathepsin B and Cathepsin L and then interacts with Niemann-Pick C1 (NPC1) in the endosomal membrane (10-12).

1. Yang, Z.-Y. et al. (2000) Nat. Med. 6:886.
2. de La Vega, M.-A. et al. (2015) Viral Immunol. 28:3.
3. Volchkov, V.E. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5762.
4. Volchkov, V.E. et al. (1998) Virology 245:110.
5. Sanchez, A. et al. (1998) J. Virol. 72:6442.
6. Dolnik, O. et al. (2004) EMBO J. 23:2175.
7. Takada, A. et al. (2004) J. Virol. 78:2943.
8. Alvarez, C.P. et al. (2002) J. Virol. 76:6841.
9. Simmons, G. et al. (2003) Virology 305:115.
10. Schornberg, K. et al. (2006) J. Virol. 80:4174.
11. Chandran, K. et al. (2005) Science 308:1643.
12. Cote, M. et al. (2011) Nature 477:344.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EBOV GP Antibody (MAB9016)(2)

Publications using MAB9016

• E Paparisto, NR Hunt, DS Labach, MD Coleman, EJ Di Gravio, MJ Dodge, NJ Friesen, M Côté, A Müller, T Hoenen, SD Barr Interferon-Induced HERC5 Inhibits Ebola Virus Particle Production and Is Antagonized by Ebola Glycoprotein Cells, 2021-09-13;10(9):. 2021-09-13 [PMID: 34572049] (Western Blot, Human)
• Y Zhang, Y Wei, Y Li, X Wang, Y Liu, D Tian, X Jia, R Gong, W Liu, L Yang IgY antibodies against Ebola virus possess post-exposure protection in a murine pseudovirus challenge model and excellent thermostability PloS Neglected Tropical Diseases, 2021-03-12;15(3):e0008403. 2021-03-12 [PMID: 33711011] (Western Blot, Hamster, Human, Primate)

Bioinformatics

• Uniprot:
  • Q05320()