EBOV GP Antibody (993408) [Alexa Fluor™ Plus 594]

• Reactivity: V
• Clone: 993408
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 594

EBOV GP Antibody (993408) [Alexa Fluor™ Plus 594] Summary

• Specificity: Detects EBOV GP in direct ELISAs.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EBOV GP Antibody (993408) [Alexa Fluor™ Plus 594]

• bovGP
• EBOV GP
• second secreted glycoprotein
• small secreted glycoprotein
• Spike glycoprotein

Background

The GP glycoprotein encoded by the genome of Ebola family viruses is a critical molecule for the pathogenicity of Ebolavirus hemorrhagic viruses (1, 2). It is processed into distinct forms for virus capsule or cell surface presentation or release from virus infected cells. The GP precursor protein is cleaved by furin at a multibasic site to yield a 140 kDa N-terminal fragment (GP1) and a 26 kDa C-terminal fragment (GP2) which remain disulfide linked (3). GP1 is entirely extracellular while GP2 is a transmembrane protein (4). Heterodimers of GP1-GP2 can further associate into trimers (5). GP expressed on virus infected cells can be shed by TACE mediated cleavage, liberating a disulfide linked complex of soluble GP1 and truncated GP2 (4-6). GP binds to multiple C-type lectins on target cell surfaces, including CLEC10A/MGL, DC-SIGN, and DC-SIGNR (7-9). Following internalization, GP1 is cleaved by Cathepsin B and Cathepsin L and then interacts with Niemann-Pick C1 (NPC1) in the endosomal membrane (10-12).

1. Yang, Z.-Y. et al. (2000) Nat. Med. 6:886.
2. de La Vega, M.-A. et al. (2015) Viral Immunol. 28:3.
3. Volchkov, V.E. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5762.
4. Volchkov, V.E. et al. (1998) Virology 245:110.
5. Sanchez, A. et al. (1998) J. Virol. 72:6442.
6. Dolnik, O. et al. (2004) EMBO J. 23:2175.
7. Takada, A. et al. (2004) J. Virol. 78:2943.
8. Alvarez, C.P. et al. (2002) J. Virol. 76:6841.
9. Simmons, G. et al. (2003) Virology 305:115.
10. Schornberg, K. et al. (2006) J. Virol. 80:4174.
11. Chandran, K. et al. (2005) Science 308:1643.
12. Cote, M. et al. (2011) Nature 477:344.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.