E. Coli O157 Antibody (9.88)

• Reactivity: Ec
• Applications: ELISA, ICC/IF
• Clone: 9.88
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

E. Coli O157 Antibody (9.88) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54353)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: A crude sonicate of E. coli O157
• Localization: Whole organism
• Specificity: This monoclonal antibody shows specificity to E.coli O157 in a simple ELISA. Escherichia coli are Gram negative bacterium that are commonly found in the lower intestine of warm-blooded organisms (endotherms). Their serological types are determined in combination with somatic antigens (O group: O1-O173) and flagella antigens (H type: H1-H56). TheE. coli that cause intestinal infectious diseases including diarrhea, acute gastritis or colitis are referred to as pathogenicE. coli, which are classified into the following 4 groups according to differences in the mode of pathogenicity; enteropathogenicE. coli(EPEC), enteroinvasiveE. coli(EIEC), enterotoxigenicE. coli(ETEC) and enterohemorrhagicE. coli(EHEC). Although the identification of pathogenicE. coli requires verification of their pathogenicity, pathogenicE. coli often have specific serotypes; therefore, typing of the serogroup and serotype is necessary in screening pathogenicE. coli.
• Isotype: IgM Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein L or PEG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1-5 ug/ml
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
• Application Notes: ELISA: For coating use Ab at 1-5ug/ml, order Ab without BSA.
  Optimal dilution for a specific application should be determined.

Reactivity Notes

E. coli O157

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein L or PEG purified

Alternate Names for E. Coli O157 Antibody (9.88)

• H7 ompC

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for E. Coli O157 Antibody (NBP2-53146)(1)

Publication using NBP2-53146

• Ontiveros-Robles JA, Villanueva-Flores F, Juarez-Moreno K et al. Antibody-Functionalized Copper Oxide Nanoparticles with Targeted Antibacterial Activity ChemistryOpen 2023-05-01 [PMID: 37226371] (In-vitro, Bacteria)