DYKDDDDK Epitope Tag Antibody (29E4.G7) [Biotin]

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Immunohistochemistry: DYKDDDDK Epitope Tag Antibody (29E4.G7) [Biotin] [NBP1-97400] - Tissue: human prostate carcinoma. A) AKT-3 antibody produced using CELLine, B) AKT-3 antibody produced using roller bottle. Fixation: ...read more
Immunohistochemistry: DYKDDDDK Epitope Tag Antibody (29E4.G7) [Biotin] [NBP1-97400] - Tissue: human lymph nodes at pH9. Fixation: formalin fixed paraffin embedded. Primary antibody: Anti-IL-6 antibody at 10 ug/mL for 1 ...read more
Immunohistochemistry: DYKDDDDK Epitope Tag Antibody (29E4.G7) [Biotin] [NBP1-97400] - Tissue was fixed with formalin and embedded in paraffin. Hematoxylin was used to counter-stain cells. A 1:100 dilution of primary ...read more

Product Details

Summary
Reactivity Hu, NASpecies Glossary
Applications WB, ELISA, Flow, IHC, IHC-P
Clone
29E4.G7
Clonality
Monoclonal
Host
Mouse
Conjugate
Biotin
Concentration
LYOPH

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DYKDDDDK Epitope Tag Antibody (29E4.G7) [Biotin] Summary

Immunogen
This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added
Specificity
The purified antibody is directed against the motif and is useful in determining its presence in various assays where the epitope tag is present at either the amino or carboxy terminus of recombinant proteins. This monoclonal tag antibody detects over-expressed proteins containing the epitope tag. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • ELISA 1:100000
  • Flow Cytometry 1:1:10 - 1:1000
  • Immunohistochemistry 1:1000-1:5000
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
This antibody is optimally suited for monitoring the expression of FLAG tagged fusion proteins. As such, this antibody can be used - identify fusion proteins containing the FLAG epitope. The antibody recognizes the epitope tag fused - either the amino- or carboxy- termini of targeted proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag.
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Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Protein A purified
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water to a final concentration of 1.0 mg/ml.

Notes

Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA)-Immunoglobulin and Protease free
Color: n/a
Conjugation Chemistry: N-hydroxysuccinimide (NHS) ester

Alternate Names for DYKDDDDK Epitope Tag Antibody (29E4.G7) [Biotin]

  • DDDDK epitope tag
  • DDK
  • DYKDDDDK Epitope Tag
  • DYKDDDDK tag
  • ECS epitope tag
  • ECS tag
  • Enterokinase Cleavage Site epitope tag
  • Enterokinase Cleavage Site tag
  • epitope tag flag
  • FLAG (FLAG is a trademark of Sigma Aldrich)
  • FLAG Tag
  • Ty 1 Tag
  • xxxDDDDK epitope tag

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for DYKDDDDK Epitope Tag Antibody (NBP1-97400). (Showing 1 - 8 of 8 FAQ).

  1. Are the FLAG Tag antibodies validated in Simple Western?
    • Yes, we offer a 2 FLAG Tag antibodies that have been tested in Simple Western: NB600-345 and NBP1-71705.
  2. Does FLAG Tag antibodies comes in lyophilized form?
    • The following FLAG Tag antibodies come in lyophilized form: NBP1-97401, NBP1-97399, NBP1-97400, NBP1-97402.
  3. What the theoretical molecular weight for FLAG Tag antibodies?
    • The TMW of FLAG Tag antibodies is approximately 150 kDa.
  4. Will FLAG Tag antibodies detect internal FLAG tags? My FLAG sequence is sandwiched between two other protein sequences.
    • If you are applying your sample(s) to SDS-PAGE, under reducing condition and follow by Western Blot, DYKDDDDK epitope tag antibodies should be able to detect the tag region located internally. However, it depends on the property of your chimeric protein and if your tag is in the hydrophobic or hydrophilic region. Although DYKDDDDK is very hydrophilic, it may not be exposed to the surface if embedded deeply inside the protein with a high content of hydrophobic amino acids with or without covalently-linked modifications such as lipid moieties.
  5. Will FLAG Tag antibodies recognizes FLAG at the C-terminal end?
    • Yes, FLAG Tag antibodies can recognize FLAG-tag at both the N- or C-terminal.
  6. Hello. We are planning to promote DYKDDDDK Antibodies in our February 2013 newsletter. Thus, we would like to check toxicity of a few products. Could you let us know whether these are lyophilized form?
    • NBP1-97400 does not come in lyophilized form.
  7. Does this FLAG Tag antibody come in lyophilized form?
    • Yes, we carry this FLAG Tag antibody in lyophilized format.
  8. What is the immunogen sequence of this FLAG Tag antibody?
    • A synthetic peptide (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) corresponding to the FLAG epitope tag peptide DYKDDDDK, conjugated to KLH.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Fluorescein Labeled NBP1-97401

Additional DYKDDDDK Epitope Tag Products

DYKDDDDK Epitope Tag NBP1-97400

Blogs on DYKDDDDK Epitope Tag.

Hoist the DYKDDDDK Epitope Tag
The DYKDDDDK Epitope Tag antibody was raised against a short, artificial sequence that is widely and commonly used as a protein fusion tag. It is pervasively present in a vast amount of scientific studies, and is a very powerful investigative research...  Read full blog post.

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