dsRNA Antibody (J5) - Azide and BSA Free

• Reactivity: All-Multi
• Applications: ELISA, ICC/IF, IHC, WB
• Clone: J5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

dsRNA Antibody (J5) - Azide and BSA Free Summary

• Description: After reconstitution antibodies should be aliquoted and stored at -20C or -70C. After adding 10 mM sodium azide undiluted antibody can also be stored at 4C for a short period of time. For long term storage the mAb should be kept frozen. Repeated freezing/thawing cycles should be avoided. When kept lyophilized the product will remain stable for at least 5 years at -20C or -70C. This ds-RNA antibody was manufactured by Scicon.
• Immunogen: Female DBA/2 mice were injected intraperitonially with a mixture of 50 ug L-dsRNA and 75 ug methylated bovine serum albumin, emulsified in complete Freund's adjuvant. After several boosts spleen cells were fused with Sp2/0-Agl4 myeloma cells to generate the hybridoma clone.
• Specificity: This antibody recognises double-stranded RNA (dsRNA) provided that the length of the helix is greater than or equal to 40 bp. dsRNA-recognition is independent of the sequence and nucleotide composition of the antigen. All naturally occurring dsRNAs investigated up to now (40-50 species) as well as poly(I).poly(C) and poly(A).poly(U) have been recognised by J5, although in some assays its affinity to poly(I).poly(C) is about 10 times lower than that to other dsRNA antigens. The antibody does not react with ssRNA, ssDNA or dsDNA.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoblotting
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
• Application Notes: This antibody can be used for ELISA, dsRNA-immunoblotting, immunoaffinity chromatography and in certain systems also for immunohistochemistry. The optimum working dilution of the antibody for any specific application should be established by titration.
  
  Please note that nucleic acid separation prior to dsRNA-immunoblotting must be carried out by polyacrylamide gel electrophoresis, because the sensitivity of detection is considerably lower after blotting from agarose gels.
  
  Not for use for clinical purposes. For in vitro use only.
  
  dsRNA-immunoblotting.

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at -20C prior to opening. Aliquot reconstituted liquid and and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing.
• Buffer: Lyophilised from PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: The lyophilised sample should be reconstituted with 200 ul (500 ul for 500 ug size) sterile distilled water. The mAb will then be in PBS without any stabilisers or preservatives at a concentration of 1 mg/ml.

Alternate Names for dsRNA Antibody (J5) - Azide and BSA Free

• Double-stranded RNA

Background

Double-stranded RNA (dsRNA)antibodies have been used extensively to detect and characterise plant and animal viruses with dsRNA genomes or intermediates. The J5 antibody can detect all tested forms of dsRNA, including poly(A):poly(U), poly(I):poly(C) and dsRNA from viruses such as Dengue Virus, Encephalomyocarditis Virus, Vaccinia Virus, Reovirus or Cucumber Mosaic Virus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.