DNA Adenine Methylase Antibody (RB1) - BSA Free Summary
| Immunogen |
Hybridoma obtained after immunization of mice with recombinant DNA Adenine Methylase protein. |
| Specificity |
The antibody reacts with the recombinant protein as produced by E.coli. We have no data to support to its reactivity with wild type DAM protein. |
| Isotype |
IgG1 |
| Clonality |
Monoclonal |
| Host |
Mouse |
| Purity |
Protein A or G purified |
| Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
| Dilutions |
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| Application Notes |
. |
Reactivity Notes
Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles. |
| Buffer |
PBS |
| Preservative |
0.08% Sodium Azide |
| Purity |
Protein A or G purified |
Alternate Names for DNA Adenine Methylase Antibody (RB1) - BSA Free
Background
DNA adenine methylase (DAM) is an enzyme that methylates adenine residues in the 4-base pair DNA sequence, GATC. This methylation reaction has been shown to be crucial in a variety of cellular processes in bacteria. These include (i) the control of initiation of DNA replication (GATC occurs 11 times in the highly conserved bacterial origin of replication sequence); (ii) methyl-directed mismatch repair; (iii) control of transposition of certain transposable DNA elements; and (iv) control of gene expression. With regard to gene expression, the recent report by Heithoff et al. (Science; 284:967-970; 1999) showed that the deletion of the DAM gene rendered Salmonella typhimurium totally avirulent.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Product General Protocols
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Secondary Antibodies
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Isotype Controls
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Additional DNA Adenine Methylase Products
Blogs on DNA Adenine Methylase
![Immunohistochemistry-Paraffin: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] [NB7539] - FFPE serial sections of human stomach carcinoma. Primary Antibody: Mouse anti-p53 (Clone DO-1) used at a dilution of 1:100. Secondary Antibody: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] Conjugated used at a dilution of 1:200 (5ug/ml). Detection: DAB](https://images.novusbio.com/images/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-HRP-Immunohistochemistry-Paraffin-NB7539-img0014.jpg "Immunohistochemistry-Paraffin: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] [NB7539] - FFPE serial sections of human stomach carcinoma. Primary Antibody: Mouse anti-p53 (Clone DO-1) used at a dilution of 1:100. Secondary Antibody: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] Conjugated used at a dilution of 1:200 (5ug/ml). Detection: DAB")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")