DMPO Nitrone Adduct Antibody (N1664A) - BSA Free

Western Blot: DMPO Nitrone Adduct Antibody (N1664A) [NBP2-59306] - Analysis of Human HL 60 clone 15 eosinophils lysates showing detection of DMPO protein using Mouse Anti-DMPO Monoclonal Antibody, Clone N1664A. Primary Antibody: Mouse Anti-DMPO Monoclonal Antibody at 1:200.

Immunocytochemistry/Immunofluorescence: DMPO Nitrone Adduct Antibody (N1664A) [NBP2-59306] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-DMPO Monoclonal Antibody, Clone N1664A (NBP2-59306). Tissue: macrophage cell line (Raw 264.7). Species: Mouse. Primary Antibody: Mouse Anti-DMPO Monoclonal Antibody (NBP2-59306) at 1:100. Secondary Antibody: Alexa Fluor 488 Goat Anti-Mouse (green) at 1:1000. Counterstain: DAPI (blue) nuclear stain.

• Reactivity: Mu, All-NA
• Applications: WB, ELISA, ICC/IF, IHC, IP, MA
• Clone: N1664A
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

DMPO Nitrone Adduct Antibody (N1664A) - BSA Free Summary

• Immunogen: 5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to Ovalbumin
• Specificity: Recognizes DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross react with non-adducted proteins or DNA.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunoprecipitation 25ug
  • Microarray
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Purity: Protein G purified

Alternate Names for DMPO Nitrone Adduct Antibody (N1664A) - BSA Free

• 2,2-dimethyl-1-oxido-3,4-dihydropyrrol-1-ium
• 2,2-Dimethyl-3,4-dihydro-2H-pyrrole 1-oxide
• 5,5-Dimethyl-1-pyrroline N-oxide
• 5,5-Dimethyl-1-pyrroline-1-oxide

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.