![Western Blot: Digoxin Antibody (26G10) [NBP1-78619] - Analysis of 10ng of BSA or Digoxin-labeled BSA per well and 5ul of PageRuler Plus Prestained Protein Ladder.](http://images.novusbio.com/fullsize/Digoxin-Antibody-26G10-Western-Blot-NBP1-78619-img0003.jpg "Western Blot: Digoxin Antibody (26G10) [NBP1-78619] - Analysis of 10ng of BSA or Digoxin-labeled BSA per well and 5ul of PageRuler Plus Prestained Protein Ladder.")
| Reactivity | All-NASpecies Glossary |
| Applications | WB, ELISA, Func, IP |
| Clone | 26G10 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Description | Novus Biologicals Mouse Digoxin Antibody (26G10) (NBP1-78619) is a monoclonal antibody validated for use in WB, ELISA and IP. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Digoxin-BSA |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Ion exchange chromatography |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.01M potassium phosphate with 0.135M NaCl, 1mg/ml BSA, 2.5mM KCl |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Ion exchange chromatography |
Secondary Antibodies |
Isotype Controls |
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![Chemiluminescence Immunoassay: Digoxin Antibody (26G10) [NBP1-78619] - Chemiluminescent detection of a Digoxin-labeled oligonucleotide was performed by diluting a Digoxin-labeled DNA oligonucleotide (+) or an unlabeled oligonucleotide control (-) in TriTracker Loading Dye, and loading 5pmols onto a 6% DNA Retardation Gel. DNA was transferred to a Biodyne B membrane and blocked for 30 minutes at room temperature. Digoxin was detected using a Digoxin monoclonal antibody at a dilution of 1ug/ml for 30 minutes at room temperature, followed by a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using the Chemiluminescent Nucleic Acid Chemiluminescent detection Module Kit.](http://images.novusbio.com/fullsize/Digoxin-Antibody-26G10-Chemiluminescence-Immunoassay-NBP1-78619-img0001.jpg "Chemiluminescence Immunoassay: Digoxin Antibody (26G10) [NBP1-78619] - Chemiluminescent detection of a Digoxin-labeled oligonucleotide was performed by diluting a Digoxin-labeled DNA oligonucleotide (+) or an unlabeled oligonucleotide control (-) in TriTracker Loading Dye, and loading 5pmols onto a 6% DNA Retardation Gel. DNA was transferred to a Biodyne B membrane and blocked for 30 minutes at room temperature. Digoxin was detected using a Digoxin monoclonal antibody at a dilution of 1ug/ml for 30 minutes at room temperature, followed by a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using the Chemiluminescent Nucleic Acid Chemiluminescent detection Module Kit.")
![ELISA: Digoxin Antibody (26G10) [NBP1-78619] - Indirect ELISA analysis of Digoxin was performed by preparing a 10ug/mL solution of BSA or Digoxin-labeled BSA in 0.2M Carbonate-Bicarbonate, pH 9.4. 100uL of each solution was added to separate wells of a clear 96-well plate, and incubated overnight at 4C. Each well was blocked with SuperBlock in PBS. The plate was then washed with PBST and incubated with 100uL per well of Digoxin monoclonal antibody in triplicate at ~1000 – 2pg/mL for 1 hour at room temperature. The plate was washed with PBST and incubated with 100ul per well of goat anti-mouse IgG-HRP secondary antibody in all test wells at 1:25,000 for 30 minutes at room temperature. Detection was performed using 1-Step TMB Ultra substrate for 15 minutes at room temperature. The plate was then stopped with 0.2M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550nm.](http://images.novusbio.com/fullsize/Digoxin-Antibody-26G10-ELISA-NBP1-78619-img0002.jpg "ELISA: Digoxin Antibody (26G10) [NBP1-78619] - Indirect ELISA analysis of Digoxin was performed by preparing a 10ug/mL solution of BSA or Digoxin-labeled BSA in 0.2M Carbonate-Bicarbonate, pH 9.4. 100uL of each solution was added to separate wells of a clear 96-well plate, and incubated overnight at 4C. Each well was blocked with SuperBlock in PBS. The plate was then washed with PBST and incubated with 100uL per well of Digoxin monoclonal antibody in triplicate at ~1000 – 2pg/mL for 1 hour at room temperature. The plate was washed with PBST and incubated with 100ul per well of goat anti-mouse IgG-HRP secondary antibody in all test wells at 1:25,000 for 30 minutes at room temperature. Detection was performed using 1-Step TMB Ultra substrate for 15 minutes at room temperature. The plate was then stopped with 0.2M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550nm.")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")