Digoxin Antibodies
We offer Digoxin Antibodies for use in common research applications: ELISA, Immunoprecipitation, Radioimmunoassay. Each Digoxin Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Digoxin Antibodies can be used in a variety of model species: Plant. Use the list below to choose the Digoxin Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Digoxin polyclonal antibodies and browse our Digoxin monoclonal antibody catalog.
Alternate Names for Digoxin Antibodies
anti-Digoxin antibody, anti-Lanoxin antibody
4 Results for "digoxin" in Primary Antibodies
![ELISA: Digoxin Antibody (PSH04-40) [NBP3-32267] - Competitive ELISA analysis of Digoxin was performed by coating wells of a 96-well plate with 50 µl per well of Digoxin-BSA diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 1% BSA blocking buffer, and incubated with 100 µl per well of Digoxin monoclonal antibody at concentration of 1 µg/mL with serial diluted Digoxin starting from a concentration of 10ug/ml for 1 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-Rabbit IgG secondary antibody at a dilution of 1/5,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](http://images.novusbio.com/fullsize/nbp3-32267_rabbit-digoxin-mab-psh04-40-27820241141611.jpg "ELISA: Digoxin Antibody (PSH04-40) [NBP3-32267] - Competitive ELISA analysis of Digoxin was performed by coating wells of a 96-well plate with 50 µl per well of Digoxin-BSA diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 1% BSA blocking buffer, and incubated with 100 µl per well of Digoxin monoclonal antibody at concentration of 1 µg/mL with serial diluted Digoxin starting from a concentration of 10ug/ml for 1 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-Rabbit IgG secondary antibody at a dilution of 1/5,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.")
![Western Blot: Digoxin Antibody (PSH04-40) [NBP3-32267] - Western blot analysis of Digoxin on different lysates with Rabbit anti-Digoxin antibody (NBP3-32267) at 1/2,000 dilution. Lane 1: DIG-BSA (2 ng/Lane) Lane 2: BSA (negative) (2 ng/Lane) Lane 3: 293T cell lysate (negative) (20 ug/Lane) Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32267) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32267_rabbit-digoxin-mab-psh04-40-278202416331224.jpg)
![Immunohistochemistry: Digoxin Antibody (PSH04-40) [NBP3-32267] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue (negative) with Rabbit anti-Digoxin antibody (NBP3-32267) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32267) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32267_rabbit-digoxin-mab-psh04-40-298202414161814.jpg)
![Western Blot: Digoxin Antibody (26G10) [NBP1-78619] - Analysis of 10ng of BSA or Digoxin-labeled BSA per well and 5ul of PageRuler Plus Prestained Protein Ladder.](http://images.novusbio.com/fullsize/Digoxin-Antibody-26G10-Western-Blot-NBP1-78619-img0003.jpg "Western Blot: Digoxin Antibody (26G10) [NBP1-78619] - Analysis of 10ng of BSA or Digoxin-labeled BSA per well and 5ul of PageRuler Plus Prestained Protein Ladder.")
![Chemiluminescence Immunoassay: Digoxin Antibody (26G10) [NBP1-78619] - Chemiluminescent detection of a Digoxin-labeled oligonucleotide was performed by diluting a Digoxin-labeled DNA oligonucleotide (+) or an unlabeled oligonucleotide control (-) in TriTracker Loading Dye, and loading 5pmols onto a 6% DNA Retardation Gel. DNA was transferred to a Biodyne B membrane and blocked for 30 minutes at room temperature. Digoxin was detected using a Digoxin monoclonal antibody at a dilution of 1ug/ml for 30 minutes at room temperature, followed by a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using the Chemiluminescent Nucleic Acid Chemiluminescent detection Module Kit.](http://images.novusbio.com/fullsize/Digoxin-Antibody-26G10-Chemiluminescence-Immunoassay-NBP1-78619-img0001.jpg)
![ELISA: Digoxin Antibody (26G10) [NBP1-78619] - Indirect ELISA analysis of Digoxin was performed by preparing a 10ug/mL solution of BSA or Digoxin-labeled BSA in 0.2M Carbonate-Bicarbonate, pH 9.4. 100uL of each solution was added to separate wells of a clear 96-well plate, and incubated overnight at 4C. Each well was blocked with SuperBlock in PBS. The plate was then washed with PBST and incubated with 100uL per well of Digoxin monoclonal antibody in triplicate at ~1000 – 2pg/mL for 1 hour at room temperature. The plate was washed with PBST and incubated with 100ul per well of goat anti-mouse IgG-HRP secondary antibody in all test wells at 1:25,000 for 30 minutes at room temperature. Detection was performed using 1-Step TMB Ultra substrate for 15 minutes at room temperature. The plate was then stopped with 0.2M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550nm.](http://images.novusbio.com/fullsize/Digoxin-Antibody-26G10-ELISA-NBP1-78619-img0002.jpg)
![Western Blot: Digoxin Antibody (38G12) [NBP1-78618] - Analysis of Digoxin was performed by loading 10ng of BSA or Digoxin-labeled BSA per well and 5ul of PageRuler Plus Prestained Protein Ladder onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Blotter, and blocked with 5% milk in TBST for 1 hour at room temperature. BSA was detected at ~66kD using a BSA monoclonal antibody at a dilution of 1ug/ml in 5% milk in TBST overnight at 4C on a rocking platform. Digoxin-labeled BSA was detected at ~66kD using a Digoxin monoclonal antibody at a dilution of 1ug/ml in 5% milk in TBST overnight at 4C on a rocking platform. After washing, blots were probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico substrate.](http://images.novusbio.com/fullsize/Digoxin-Antibody-38G12-Western-Blot-NBP1-78618-img0003.jpg "Western Blot: Digoxin Antibody (38G12) [NBP1-78618] - Analysis of Digoxin was performed by loading 10ng of BSA or Digoxin-labeled BSA per well and 5ul of PageRuler Plus Prestained Protein Ladder onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Blotter, and blocked with 5% milk in TBST for 1 hour at room temperature. BSA was detected at ~66kD using a BSA monoclonal antibody at a dilution of 1ug/ml in 5% milk in TBST overnight at 4C on a rocking platform. Digoxin-labeled BSA was detected at ~66kD using a Digoxin monoclonal antibody at a dilution of 1ug/ml in 5% milk in TBST overnight at 4C on a rocking platform. After washing, blots were probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico substrate.")
![Chemiluminescence Immunoassay: Digoxin Antibody (38G12) [NBP1-78618] - Chemiluminescent detection of a Digoxin-labeled oligonucleotide was performed by diluting a Digoxin-labeled DNA oligonucleotide (+) or an unlabeled oligonucleotide control (-) in TriTracker Loading Dye, and loading 5pmols onto a 6% DNA Retardation Gel. DNA was transferred to a Biodyne B membrane and blocked for 30 minutes at room temperature. Digoxin was detected using a Digoxin monoclonal antibody at a dilution of 1ug/ml for 30 minutes at room temperature, followed by a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using the Chemiluminescent Nucleic Acid Chemiluminescent detection Module Kit.](http://images.novusbio.com/fullsize/Digoxin-Antibody-38G12-Chemiluminescence-Immunoassay-NBP1-78618-img0001.jpg)
![ELISA: Digoxin Antibody (38G12) [NBP1-78618] - InDirect ELISA analysis of Digoxin was performed by preparing a 10ug/mL solution of BSA or Digoxin-labeled BSA in 0.2M Carbonate-Bicarbonate, pH 9.4. 100uL of each solution was added to separate wells of a clear 96-well plate, and incubated overnight at 4C. Each well was blocked with SuperBlock in PBS. The plate was then washed with PBST and incubated with 100uL per well of Digoxin monoclonal antibody in triplicate at ~1000 – 2pg/mL for 1 hour at room temperature. The plate was washed with PBST and incubated with 100ul per well of goat anti-mouse IgG-HRP secondary antibody in all test wells at 1:25,000 for 30 minutes at room temperature. Detection was performed using 1-Step TMB Ultra substrate for 15 minutes at room temperature. The plate was then stopped with 0.2M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550nm.](http://images.novusbio.com/fullsize/Digoxin-Antibody-38G12-ELISA-NBP1-78618-img0002.jpg)
![Dot Blot: Digoxin Antibody (7H3V0) [NBP3-35056] - The Digoxin Rabbit mAb are tested in Dot Blot against digoxin labelled oligonucleotide(Dig-1，Dig-2 and Dig-3) and unlabelled oligonucleotide(Dig-4) Dig-1 :5'AGCTAAC/iDigdT/ACTAGCT(Biotin)3' Dig-2 :5'(Digoxin)AGCTAACTACTAGCT(Biotin)3' Dig-3 :5'(Biotin)AGCTAACTACTAGCT(Digoxin)3' Dig-4 :5'AGCTAACTACTAGCT(Biotin)3'](http://images.novusbio.com/fullsize/nbp3-35056_rabbit-digoxin-mab-7h3v0-10122024140435.jpg "Dot Blot: Digoxin Antibody (7H3V0) [NBP3-35056] - The Digoxin Rabbit mAb are tested in Dot Blot against digoxin labelled oligonucleotide(Dig-1，Dig-2 and Dig-3) and unlabelled oligonucleotide(Dig-4) Dig-1 :5'AGCTAAC/iDigdT/ACTAGCT(Biotin)3' Dig-2 :5'(Digoxin)AGCTAACTACTAGCT(Biotin)3' Dig-3 :5'(Biotin)AGCTAACTACTAGCT(Digoxin)3' Dig-4 :5'AGCTAACTACTAGCT(Biotin)3'")
![ELISA: Digoxin Antibody (7H3V0) [NBP3-35056] - The Digoxin Rabbit mAb are tested in ELISA against digoxin labelled oligonucleotide(Dig-1，Dig-2 and Dig-3) and unlabelled oligonucleotide(Dig-4) Dig-1 :5'AGCTAAC/iDigdT/ACTAGCT(Biotin)3' Dig-2 :5'(Digoxin)AGCTAACTACTAGCT(Biotin)3' Dig-3 :5'(Biotin)AGCTAACTACTAGCT(Digoxin)3' Dig-4 :5'AGCTAACTACTAGCT(Biotin)3'](http://images.novusbio.com/fullsize/nbp3-35056_rabbit-digoxin-mab-7h3v0-10122024140422.jpg)