Dendritic Cell Marker Antibody (33D1) - BSA Free

Flow Cytometry: Dendritic Cell Marker Antibody (33D1) [NBP1-43284] - Analysis using the PE conjugate of NBP1-43284. Staining of BALB/c splenocytes with Anti-Mouse MHC Class II (I-A/I-E) FITC and 0.125 ug of Rat IgG2b kappa Isotype Control PE (left) or 0.125 ug of Anti-Mouse Dendritic Cell Marker (33D1) PE (right).

Flow Cytometry: Dendritic Cell Marker Antibody (33D1) [NBP1-43284] - Staining of BALB/c splenocytes with 0.25 ug of Rat IgG2b kappa Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse Dendritic Cell Marker (33D1) Purified (filled histogram) followed by Anti-Rat IgG Biotin and Streptavidin PE . Total viable c

Flow Cytometry: Dendritic Cell Marker Antibody (33D1) [NBP1-43284] - Analysis using the Biotin conjugate of NBP1-43284. Staining of C57Bl/6 splenocytes with Anti-Mouse MHC Class II (I-A/I-E) FITC and 0.25 ug of Rat IgG2b Isotype Control biotin (left) or 0.25 ug of bnti-Mouse Dendritic Cell Marker (33D1) Biotin (right) followed by Streptavidin PE.

• Reactivity: Mu
• Applications: Flow, IHC
• Clone: 33D1
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

Dendritic Cell Marker Antibody (33D1) - BSA Free Summary

• Description: The 33D1 monoclonal antibody reacts with a mouse dendritic cell (DC)-specific surface marker, DCIR2. The nature and biological activity of DCIR2 is unknown. DCIR2 has been reported on a variety of dendritic cell subpopulations from mouse thymus, spleen, lymph node, and Peyer's patch. Bone marrow dendritic cells require GM-CSF to express DCIR2, and this expression is downregulated in the presence of IL-4. DCIR2 has been detected in vivo in brain dendritic cells post infection with Toxoplasma gondii.
• Immunogen: The immunogen for this antibody was Dendritic Cell Marker.
• Marker: Dendritic Cell Marker
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Rat
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
• Application Notes: The 33D1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for Dendritic Cell Marker Antibody (33D1) - BSA Free

• DCs
• Dendritic cell

Background

The 33D1 monoclonal antibody reacts with a mouse DC-specific surface marker. The nature and biological activity of the 33D1 antigen is as of yet unknown. 33D1 has been reported on a variety of dendritic cell subpopulations from mouse thymus, spleen, lymph node, and Peyers patch. Bone marrow dendritic cells require GM-CSF to express the 33D1 antigen and this expression is down regulated in the presence of IL-4. 33D1 antigen has been detected in vivo in brain dendritic cells post infection via Toxoplasma gondii.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Dendritic Cell Marker Antibody (NBP1-43284)(1)

Publication using NBP1-43284

• McLellan AD, Kapp M, Eggert A et al. Anatomic location and T-cell stimulatory functions of mouse dendritic cell subsets defined by CD4 and CD8 expression. Blood. 2002-03-15 [PMID: 11877283] (FLOW, Mouse)
  
  Details:
  This citation used the FITC version of this antibody.