DDX19A/B Antibody

Western Blot: DDX19A/B Antibody [NB100-68149] - (0.5ug/ml) Analysis of K562 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

DDX19A/B Antibody Summary

• Description: Novus Biologicals Goat DDX19A/B Antibody (NB100-68149) is a polyclonal antibody validated for use in WB and ELISA. Anti-DDX19A/B Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-DLPVDKDGNPDNETY corresponding to internal region according to NP_001014451.1, NP_001014449.1, NP_060802.1.
• Epitope: C-DLPVDKDGNPDNETY
• Specificity: This antibody is expected to recognise DDX19A (NP_060802.1) and all reported isoforms of DDX19B (NP_009173.1, NP_001014451.1 and NP_001014449.1).
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DDX19A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 0.5 - 1.5 ug/ml
• Application Notes: WB: Approx. 50 kDa band observed in lysates of human ovary and K562 (calculated MW of 50.5 kDa band according to NP_001014451.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DDX19A/B Antibody

• DDX19A DEAD (Asp-Glu-Ala-Asp) box polypeptide 19A
• DDX19-DDX19L
• DDX19L

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which exhibits RNA-dependent ATPase and ATP-dependent RNA-unwinding activities. This protein is recruited to the cytoplasmic fibrils of the nuclear pore complex, where it participates in the export of mRNA from the nucleus. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DDX19A/B Antibody (NB100-68149)(1)

Publication using NB100-68149

• Cole CN, Scarcelli JJ. Transport of messenger RNA from the nucleus to the cytoplasm. Curr Opin Cell Biol 2006-06-01 [PMID: 16682182]

Bioinformatics

• Gene Symbol: DDX19A
• Entrez:
  • 11269(Human)
  • 234733(Mouse)
  • 690693(Rat)
• Uniprot:
  • NP_009173.1(Human)