![ELISA: D-Dimer Antibody (28) - BSA Free [NBP1-05045] - The calibration curve of a sandwich assay for D-Dimer using NBP1-05045 as capture antibody and NBP2-23592 as the biotinylated detection antibody.](http://images.novusbio.com/fullsize/D-Dimer-Antibody-28-BSA-Free-ELISA-NBP1-05045-img0004.jpg "ELISA: D-Dimer Antibody (28) - BSA Free [NBP1-05045] - The calibration curve of a sandwich assay for D-Dimer using NBP1-05045 as capture antibody and NBP2-23592 as the biotinylated detection antibody.")
| Reactivity | Hu, CaSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clone | 28 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Description | Novus Biologicals Mouse D-Dimer Antibody (28) - BSA Free (NBP1-05045) is a monoclonal antibody validated for use in IHC, WB and ELISA. Anti-D-Dimer Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Purified human D-dimer |
| Epitope | The epitope appears to relate to the neo-epitope formed by cleavage of D-dimer from fibrin. |
| Specificity | Binds human D-dimer. Cross-reacts with directly coated D-monomer but not with directly
coated fibrinogen. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | ELISA: NBP1-05045 binds free D-dimer in solution. NBP1-05045 works as a capture antibody in sandwich ELISA with biotinylated NBP1-05045 or NBP2-21616 as a detection antibody. Biotinylated NBP1-05045 works as a detection antibody in sandwich ELISA with NBP2-21616 or NBP1-05045 as a capture antibody. The combination with NBP1-05045 as a capture antibody and NBP2-23592 as a detection antibody is recommended.
WB: Due to reducing conditions in Western blotting NBP1-05045 binds the beta-chain (40kDa) of D-Dimer, and not the entire D-Dimer complex. Use in IHC-P reported in scientific literature (PMID:33480806) |
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| Publications |
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| Storage | Store at 4C in the dark. |
| Buffer | 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
| Publication using NBP1-05045 | Applications | Species |
|---|---|---|
| Pellegrini D, Kawakami R, Guagliumi G, et al. Microthrombi As A Major Cause of Cardiac Injury in COVID-19: A Pathologic Study Circulation 2021-01-22 [PMID: 33480806] (IHC-P, Human) | IHC-P | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for D-Dimer Antibody (NBP1-05045)Find related products by research area.
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")