Cytomegalovirus Antibody (DDG9 and CCH2)

Immunohistochemistry-Paraffin: Cytomegalovirus Antibody (DDG9 and CCH2) [NBP1-22532] - IHC analysis of a formalin fixed paraffin embedded tissue section of CMV infected lung using Cytomegalovirus antibody (clone DDG9 and CCH2).

Immunohistochemistry: Cytomegalovirus Antibody (DDG9 and CCH2) [NBP1-22532] - Human Placenta CMV. HIER sodium citrate 6.0 - 30 minutes 1:10 dilution. This image was submitted via customer Review.

Immunohistochemistry-Paraffin: Cytomegalovirus Antibody (DDG9 and CCH2) [NBP1-22532] - IHC analysis of a formalin fixed paraffin embedded tissue section of CMV infected lung using Cytomegalovirus antibody (clone DDG9 and CCH2).

Immunohistochemistry-Paraffin: Cytomegalovirus Antibody (DDG9 and CCH2) [NBP1-22532] - IHC analysis of a formalin fixed paraffin embedded human salivary glands (CMV infected) using Cytomegalovirus antibody (clone DDG9 and CCH2).

• Reactivity: V, V-Vi
• Applications: IHC, IHC-P
• Clone: DDG9 and CCH2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Cytomegalovirus Antibody (DDG9 and CCH2) Summary

• Immunogen: BALB/C mice were immunized with cytomegalovirus infected cell lysate. This antibody is a cocktail of DDG9 and CCH2.
• Localization: Nuclear
• Specificity: This cocktail antibody reacts with an immediate early and early CMV antigens. The antibody stains infected cells giving a nuclear staining pattern with an immediate early and an early antigen.
• Isotype: IgG1 Kappa/IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:25
  • Immunohistochemistry-Paraffin 1:10-1:25
• Application Notes: IHC-P: recommended pretreatment of citrate buffer, pH 6.0. Recommended incubation time of 30 min at RT.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tissue culture supernatant
• Preservative: 0.05% Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for Cytomegalovirus Antibody (DDG9 and CCH2)

• CMV; HHV 5; HHV5; Human herpesvirus 5

Background

Human cytomegalovirus, also called HCMV or human herpesvirus 5, is a double-stranded DNA, enveloped virus of 230-250 kilobases (kb) that is a member of the Herpesviridae family and the subfamily Betaherpesvirinae (1,2). Other herpesviruses, which share the characteristic of lifelong latency following primary infection, include herpes simplex virus types 1 (HSV-1) and 2 (HSV-2), varicella zoster virus (VSV), and Epstein-Barr virus (EBV) (1). Cytomegalovirus entry into cells is mediated by a trimeric complex of glycoproteins gH, gL, and gO and a pentamer made of proteins gH, gL, UL128, UL130, and UL131A (1,3). The trimer binds PDGFRalpha for entry into fibroblasts while the pentamer binds neuropilin-2 for entry into endothelial and epithelial cells (1,4). Additionally, the fusion protein glycoprotein B (gB) plays a pivotal role for cytomegalovirus entry into the host cell membrane (1,3).

Cytomegalovirus is a common virus, infecting ~60% of adults in developed countries and over 90% in developing countries, as indicated by presence of specific IgG antibodies (2-4). Although a majority of people infected remain asymptomatic, immunocompromised people, organ transplant recipients, and fetuses and newborns are more susceptible to HCMV-associated diseases (1-4). Annually, up to 2% of newborns are born with HCMV, making it the most common congenital infection (2-4). Cytomegalovirus is known to cause sensorineural hearing loss (SNHL), mental retardation, chorioretinitis, skin lesions, as well as end organ disease (3,5). There are a few CMV therapeutics approved by FDA including valganciclovir and letermovir; however, despite some benefits, there is the need for new antivirals and combination therapies (5). Potential new treatment options include monoclonal antibodies and sirtuins (5).

References

1. Connolly SA, Jardetzky TS, Longnecker R. The structural basis of herpesvirus entry. Nat Rev Microbiol. 2021;19(2):110-121. https://doi.org/10.1038/s41579-020-00448-w

2. Griffiths P, Reeves M. Pathogenesis of human cytomegalovirus in the immunocompromised host. Nat Rev Microbiol. 2021;19(12):759-773. https://doi.org/10.1038/s41579-021-00582-z

3. Krstanovic F, Britt WJ, Jonjic S, Brizic I. Cytomegalovirus Infection and Inflammation in Developing Brain. Viruses. 2021;13(6):1078. Published 2021 Jun 4. https://doi.org/10.3390/v13061078

4. Griffiths P, Baraniak I, Reeves M. The pathogenesis of human cytomegalovirus. J Pathol. 2015;235(2):288-297. https://doi.org/10.1002/path.4437

5. Acosta E, Bowlin T, Brooks J, et al. Advances in the Development of Therapeutics for Cytomegalovirus Infections. J Infect Dis. 2020;221(Suppl 1):S32-S44. https://doi.org/10.1093/infdis/jiz493

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Reviews for Cytomegalovirus Antibody (NBP1-22532) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Verified Customer IHC Human 07/07/2017

Summary

• Application: Immunohistochemistry
• Sample Tested: 5um paraffin,Placental tissue
• Species: Human
• Lot: G156

Comments

• Comments: HIER sodium citrate 6.0 - 30 minutes
  1:10 dilution

reviewed by: Nick Van Sciver WB Human 11/16/2016

Summary

• Application: Western Blot
• Sample Tested: Gingival cells,Human Oral Keratinocyte
• Species: Human
• Lot: N/A