Cytochrome P450 3A7 Antibody (C1) - BSA Free

Immunohistochemistry-Paraffin: Cytochrome P450 3A7 Antibody (C1) [NBP3-42172] - DAB staining on IHC-P; Sample: Human Liver Tissue; Primary Ab: 20ug/ml NBP3-42172; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody

• Reactivity: Hu
• Applications: IHC
• Clone: C1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Cytochrome P450 3A7 Antibody (C1) - BSA Free Summary

• Immunogen: Immunized with recombinant Human Cytochrome P450 3A7 (Uniprot#: P24462; Pro344-Asp497 (Uniprot#: P24462)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-20ug/mL
  • Immunohistochemistry-Paraffin 5-20ug/mL

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.01M PBS, pH7.4, 50% glycerol
• Preservative: 0.05% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Cytochrome P450 3A7 Antibody (C1) - BSA Free

• Aryl hydrocarbon hydroxylase
• CP37
• CYPIIIA7
• Cytochrome P450 3A7
• cytochrome P450
• family 3
• Flavoprotein linked monooxygenase
• Microsomal monooxygenase
• P450 HFLA
• polypeptide 7
• subfamily A
• subfamily IIIA
• Xenobiotic monooxygenase

Background

The Cytochrome P450 (P450) family of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as NADPH and P450 reductase. There are over 200 cDNA®s which encode P450 protein. Epoxygenases are those P450 proteins which metabolize AA to epoxyeicosatrienoic acid (EETs) and w-hydroxylases are those P450 proteins which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). 20-HETE is converted from AA by the 4A family of P450 proteins which includes at least 8 different, though closely related isoforms. 4A1, 4A2, & 4A3 have been cloned from liver, kidney and testis and have been detected in renal, hepatic & brain microvessels.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.