CRISPR-Cpf1 Antibody (2D5-6G11)

Images

 
Western Blot: CRISPR-Cpf1 Antibody (2D5-6G11) [NBP2-78819] - Lachnospiracea Cpf1 protein and negative control protein from human HeLa and mouse Neuro2A was separated on a 4-20% gel by SDS-PAGE, transferred to PVDF ...read more

Product Details

Summary
Reactivity Ba, BaSpecies Glossary
Applications WB
Clone
2D5-6G11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

CRISPR-Cpf1 Antibody (2D5-6G11) Summary

Immunogen
Partial recombinant protein made to an N-terminal portion of the Lachnospiraceae bacterium CRISPR-Cpf1 protein
Specificity
This antibody is specific for Lachnospiraceae bacterium CRISPR-Cpf1.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for CRISPR-Cpf1 Antibody (2D5-6G11)

  • anti-cas12a antibody
  • anti-cas12a
  • anti-CPF1
  • anti-CRISPR-Cas12a
  • anti-CRISPR-Cpf1
  • Cas12a
  • Clustered regularly interspaced short palindromic repeat (CRISPR) associated Cas12a
  • Cpf1
  • CRISPR-associated endonuclease Cas12a
  • CRISPR-associated endonuclease Cpf1
  • CRISPR-Cas12a
  • FnCas12a
  • FnCpf1

Background

Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) is an adaptive immune system in bacteria which provides protection against mobile genetic elements from viruses such as, transposable elements and conjugative plasmids. CRISPR clusters are known to have sequences which are complementary to antecedent mobile elements and target invading nucleic acids, and these sequences are transcribed/processed into CRISPR RNA. CRISPR possess endonuclease activity on pre-crRNA and dsDNA, using different active sites, and in a complexed form, crRNA displaces the non-target DNA strand and guides endo-nucleolytic cleavage of both strands of the DNA. CRISPR associated Cas12a (CRISPR-Cas12a), also known as Cpf1, is emerging as a genome editing tool with different target specificity, when compared to CRISPR-Cas9. CRISPR-Cas12a-assisted recombineering can be used for genetic manipulation of plasmids, plasmid curing and for a more precise selection of target sites for the introduction of desired mutations (point mutations, deletions, insertions, and replacements). In cells subjected to genetic manipulation using this tool, CRISPR-Cas12a antibodies are needed to verify transfection efficiency, CRISPR-Cas12a expression, CRISPR-Cas12a target binding and subcellular localization. Novus Biologicals' CRISPR-Cpf1 antibody (NBP2-62735) is the first-ever commercially launched antibody to meet this need. This antibody is generated against a partial recombinant Francisella tularensis protein (between amino acids 600-850) [NCBI WP_003034647.1], and validated for Western blot using a partial recombinant CRISPR-Cpf1 protein (12kDa), making it a great tool for a direct detection of CRISPR-Cpf1/CRISPR-Cas12a complex (at protein level) superior to the use of epitope-tag based validation approaches.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CRISPR-Cpf1 Antibody (NBP2-78819) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-78819AF350
Alexa Fluor 405 NBP2-78819AF405
Alexa Fluor 488 NBP2-78819AF488
Alexa Fluor 532 NBP2-78819AF532
Alexa Fluor 594 NBP2-78819AF594
Alexa Fluor 647 NBP2-78819AF647
Alexa Fluor 700 NBP2-78819AF700
Alexa Fluor 750 NBP2-78819AF750
Biotin NBP2-78819B
DyLight 350 NBP2-78819UV
DyLight 405 NBP2-78819V
DyLight 488 NBP2-78819G
DyLight 550 NBP2-78819R
DyLight 594 NBP2-78819DL594
DyLight 650 NBP2-78819C
DyLight 680 NBP2-78819FR
DyLight 755 NBP2-78819IR
HRP NBP2-78819H

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Research Areas for CRISPR-Cpf1 Antibody (NBP2-78819)

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