Cecropin B Antibody - BSA Free

• Reactivity: Sw
• Applications: WB, ELISA, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Cecropin B Antibody - BSA Free Summary

• Description: This product was prepared from monospecific antiserum by immunoaffinity chromatography using cecropin B coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified cecropin B
  
  Store antibody at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: This affinity purified Cecropin B Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic full-length Cecropin B corresponding to a modified sequence from that which is found in silkworm: Lys-Trp-Lys-Val-Phe-Lys-Lys-Ile-Glu-Lys-Val-Gly-Arg-Asn-Ile-Arg-Asn-Gly-Ile-Val-Lys-Ala-Gly-Pro-Ala-Ile-Ala-Val-Leu-Gly-Glu-Ala-Lys-Ala-Leu. (Uniprot: P04142)
• Specificity: Reactivity against other forms of cecropins has not yet been determined.
• Isotype: Serum
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:20000
  • Immunoprecipitation 1:100
  • Western Blot 1:500-1:5000
• Application Notes: This product is useful for the detection of cecropin B in various immunological assays. This reagent has specific value for the study of cecropins in transgenic plants. The antibody may also recognize naturally occurring cecropin A. Cecropin B has been modified in some instances by substituting a single amino acid to render the molecule less susceptible to proteolytic cleavage.

Reactivity Notes

Reactivity against other forms of cecropins has not yet been determined.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 50% (v/v) Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Cecropin B Antibody - BSA Free

• CECB1
• CECB2
• Lepidopteran A/B

Background

Cecropins are produced by insects, particularly under conditions of infection. Cecropins are bioactive peptides that exhibit activities by interacting with membranes and forming transmembrane channels that allow the free flow of electrolytes, metabolites and water across the phospholipid bilayers. Cecropins A, B and D are close homologues consisting of 35-39 residues. They are found in the pupae of the cecropin moth, but related homologues named lepodopteran, bactericidin, moricin and sarcotoxin are produced by other insects.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cecropin B Antibody (NB600-867)(1)

Publication using NB600-867

• Lee SJ, Yu JS, Parker BL et al. Production of antibacterial Bombyx mori cecropin A in mealworm-pathogenic Beauveria bassiana ERL1170. J. Ind. Microbiol. Biotechnol. 2015-01-01 [PMID: 25429897] (WB, Silkworm)
  
  Details:
  Cecropin B antibody used in WB for the detection of cecropin A expression - secreted from the mycelia of the transformants D-6 of mealworm-pathogenic Beauveria bassiana ERL1170 (expressing Bombyx mori cecropin A). WB data of cecropin A along with RT-PCR and SDS-PAGE results is shown in Fig 2 of this article.

Bioinformatics

• Uniprot:
  • P04142(Silkworm)