CEACAM19 Antibody (1042108) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with (A) human CEACAM-19 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human CEACAM-19 Monoclonal Antibody (Catalog # MAB93971) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on Mouse IgG1 Isotype Control (MAB002). Staining was performed using our Staining Membrane-associated Proteins protocol.

• Reactivity: Hu
• Applications: Flow
• Clone: 1042108
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CEACAM19 Antibody (1042108) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human CEACAM-19
  Met1-Gly157
  Accession # Q7Z692
• Specificity: Detects human CEACAM-19 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CEACAM19 Antibody (1042108) [Unconjugated]

• carcinoembryonic antigen-related cell adhesion molecule 19
• CEACAM19
• CEACAM-19
• CEAL1

Background

Carcinoembryonic antigen-related cell adhesion molecule-19 (CEACAM-19), also known as CEA-like gene 1 (CEAL1), is a member of the CEACAM subfamily of glycoproteins in the immunoglobulin (Ig) superfamily. Mature human CEACAM-19 consists of a 125 amino acid (aa) extracellular domain, a 21 aa helical transmembrane domain, and a 122 aa cytoplasmic domain (1,2). The extracellular domain contains an Ig-like domain as well as a glycosylation site, while the cytoplasmic domain is predicted to contain at least one immunoreceptor tyrosine-based activation motif (ITAM) (3,4). The extracellular domain of human CEACAM-19 shows 71%, 72%, and 78% aa identity to mouse, rat, and bovine CEACAM-19. The CEACAM family of proteins are involved in numerous intercellular-adhesion and intracellular signaling processes including cell adhesion, cell growth, recognition and differentiation, angiogenesis, and apoptosis (5,6). CEACAM-19 expression has been identified in a wide range of tissues including prostate, uterus, fetal brain, mammary and adrenal glands, skeletal muscle, small intestine, and kidney (1). Human CEACAM-19 has been found to be overexpressed in BT-474, BT20, and T47D breast cancer cell lines as well as LNCaP prostate cancer cells, suggesting a role in tumor progression and metastasis (1,3, 7). The overexpression in breast cancer patients is associated with poor prognosis (7).

1. Schorilas, A. et al. (2003) Gene 310:79.
2. Estiar, M. et al. (2016) Clin Exp Med doi:10.1007/s10238-016-0442-1.
3. Beauchemin, N. and Arabzadeh, A. (2013) Cancer Metastasis Rev 32 (3-4):643.
4. Kuespert, K. et al. (2006) Curr Opin Cell Biol 18:565.
5. Obrink, B. (1997) Curr Opin Cell Biol 9:616.
6. Horst, AK. and Wagener, C. (2004) Handb Exp Pharmacol 283.
7. Michaelidou, K. et al. (2013) Int J Oncol 42:1770.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q7Z692()