Caspase-11/Caspase-4 Antibody (1172C) [Alexa Fluor® 700]

• Reactivity: Mu
• Applications: WB
• Clone: 1172C
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Alexa Fluor 700

Caspase-11/Caspase-4 Antibody (1172C) [Alexa Fluor® 700] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: HEK293 human embryonic kidney cell line transfected with mouse Caspase-11/Caspase-4
  Accession # P70343
• Specificity: Detects mouse Caspase-11/Caspase-4 in Western blots.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Caspase-11/Caspase-4 Antibody (1172C) [Alexa Fluor® 700]

• CASP11
• Casp11;CASP-11;Casp4;CASP-4;Caspl;Ich3;ich-3
• Caspase-11
• Caspl
• Ich-3

Background

Mouse Caspase-11 (commonly referred as Caspase-4) is a member of the cysteine-aspartic acid protease family known as caspases that function in initiating apoptosis or activating proinflammitory cytokines.   Mouse Caspase-11 is expressed as a 343 amino acid latent zymogen.  The active caspase-11 is cleaved into two subunits, p20 (aa 81-266) and p10 (aa 286-373).  Caspase-11 is one of several caspases associated with the inflammasome and plays an important role in the activation of proinflammatory cytokines.   Caspase-11 is activated by an autocatalytic mechanism or by cleavage by Caspase-8 in response to ER stress or bacterial infection, and its expression and activation can be induced by exposure to LPS.  The active enzyme can cleave and activate Caspase-1 which in turn activates IL-1 beta.   Mouse Caspase-11 has a 59 and 89% homology to human and rat Caspase-4, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.