| Reactivity | Ca, ViSpecies Glossary |
| Applications | WB, ELISA, IHC, IHC-Fr, IHC-P |
| Clone | DV2-12 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Made to Viral CANINE DISTEMPER VIRUS |
| Specificity | NB100-64816 recognizes canine distemper virus nucleoprotein. In Western blots the antibody recognizes bands of 76kD, 68kD, 54kD and 32kD under reducing conditions. This antibody is useful for the detection of CDV in immunofluorescence procedures, in which the staining seen has a cytoplasmic pattern in infected cells. It is also effective in paraffin embedded material. This antibody is anti morbillivirus specific and reacts with phocine distemper virus, racoon distemper virus and human measles virus. It does not cross react with CAV2, CCV, CPI or CPV. |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Phosphate buffered saline |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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![Flow Cytometry: Mouse IgG2b Isotype Control (MPC-11) [NBP2-27231] - An intracellular stain was performed on Daudi cells with FoxO1/FKHR (83N7F8) antibody NBP2-31376AF488 (blue) and a matched isotype control NBP2-27231AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488. Image using the Alexa Fluor 488 form of this antibody.](http://images.novusbio.com/images2/Mouse-IgG2b-Isotype-Control-(MPC-11)-Flow-Cytometry-NBP2-27231-img0003.jpg "Flow Cytometry: Mouse IgG2b Isotype Control (MPC-11) [NBP2-27231] - An intracellular stain was performed on Daudi cells with FoxO1/FKHR (83N7F8) antibody NBP2-31376AF488 (blue) and a matched isotype control NBP2-27231AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488. Image using the Alexa Fluor 488 form of this antibody.")