![Immunohistochemistry-Paraffin: CA19-9/Sialyl Lewis A Antibody (SPM588) - IHC-Prediluted [NBP2-48432] - Human Colon Carcinoma stained with CA19-9 Monoclonal Antibody (SPM588)).](http://images.novusbio.com/fullsize/CA19-9-Sialyl-Lewis-A-Antibody-SPM588-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48432-img0001.jpg "Immunohistochemistry-Paraffin: CA19-9/Sialyl Lewis A Antibody (SPM588) - IHC-Prediluted [NBP2-48432] - Human Colon Carcinoma stained with CA19-9 Monoclonal Antibody (SPM588)).")
| Reactivity | HuSpecies Glossary |
| Applications | IHC |
| Clone | SPM588 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | IHC-Prediluted |
| Description | The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining. |
| Immunogen | Precipitin lines obtained after immuno-diffusion using MAb 116-NS-19-9 and mucins isolated from an ovarian cyst of a Lewis A+B- patient (0Le). |
| Localization | Cytoplasmic |
| Marker | GI Tumor Marker |
| Specificity | CA19-9, a carbohydrate epitope expressed on a high MW (400kDa) mucin glycoprotein, is a sialyl Lewisa structure which is synthesized from type 1 blood group precursor chains and is present in individuals expressing the Lewisa and/or Lewisb blood group antigens. In normal tissues, sialyl Lewisa antigen is present in ductal epithelium of the breast, kidney, salivary gland, and sweat glands. Its expression is greatly enhanced in serum as well as in the majority of tumor cells in gastrointestinal (GI) carcinomas, including adenocarcinomas of the stomach, intestine, and pancreas. Preoperative elevated CA19-9 levels in patients with stage I pancreatic carcinoma decrease to normal values following surgery. When used serially, CA19-9 can predict recurrence of disease prior to radiographic or clinical findings. This monoclonal antibody is superb for staining of formalin-fixed, paraffin-embedded tissues. |
| Isotype | IgM Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein L or PEG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Hu-chromosome location: 1q32.2 Molecular weight of antigen: 90-110kDa Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application. |
| Theoretical MW | 400 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein L or PEG purified |
Secondary Antibodies |
Isotype Controls |
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