CA19-9/Sialyl Lewis A Antibody (SPM588)

Immunohistochemistry-Paraffin: CA19-9/Sialyl Lewis A Antibody (SPM588) [NBP2-45199] - Human thyroid carcinoma tissue section. Fixed with 4% formaldehyde, incubated O/N at 4C with primary antibody. IHC-P image submitted by a verified customer review.

Immunohistochemistry-Paraffin: CA19-9/Sialyl Lewis A Antibody (SPM588) [NBP2-45199] - Human Colon Carcinoma stained with CA19-9 Monoclonal Antibody (SPM588)).

• Reactivity: Hu
• Applications: Flow, ICC/IF, IHC, ICC/IF
• Clone: SPM588
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

CA19-9/Sialyl Lewis A Antibody (SPM588) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP3-11589)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Precipitin lines obtained after immuno-diffusion using MAb 116-NS-19-9 and mucins isolated from an ovarian cyst of a Lewis A+B- patient (0Le).
• Localization: Cytoplasmic
• Marker: GI Tumor Marker
• Specificity: CA19-9, a carbohydrate epitope expressed on a high MW (400kDa) mucin glycoprotein, is a sialyl Lewisa structure which is synthesized from type 1 blood group precursor chains and is present in individuals expressing the Lewisa and/or Lewisb blood group antigens. In normal tissues, sialyl Lewisa antigen is present in ductal epithelium of the breast, kidney, salivary gland, and sweat glands. Its expression is greatly enhanced in serum as well as in the majority of tumor cells in gastrointestinal (GI) carcinomas, including adenocarcinomas of the stomach, intestine, and pancreas. Preoperative elevated CA19-9 levels in patients with stage I pancreatic carcinoma decrease to normal values following surgery. When used serially, CA19-9 can predict recurrence of disease prior to radiographic or clinical findings. This monoclonal antibody is superb for staining of formalin-fixed, paraffin-embedded tissues.
• Isotype: IgM Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein L or PEG purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1 - 2 ug/mL for 30 minutes at RT. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 400 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein L or PEG purified

Alternate Names for CA19-9/Sialyl Lewis A Antibody (SPM588)

• CA 19-9
• CA19-9

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for CA19-9/Sialyl Lewis A Antibody (NBP2-45199) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IHC-P Human 10/14/2021

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Thyroid carcinoma
• Species: Human
• Lot: SPM588

Comments

• Comments: 4% formaldehyde fixed paraffin sections, incubated O/N in 4 C with primary antibody.