![Western Blot: CA19-9/Sialyl Lewis A Antibody (GT174) [NBP3-13649] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CA19-9/Sialyl Lewis A antibody [GT174] (NBP3-13649) diluted at 1:1000 and competitor's antibody diluted at 1:250. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CA19-9-Sialyl-Lewis-A-Antibody-GT174-Western-Blot-NBP3-13649-img0004.jpg "Western Blot: CA19-9/Sialyl Lewis A Antibody (GT174) [NBP3-13649] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CA19-9/Sialyl Lewis A antibody [GT174] (NBP3-13649) diluted at 1:1000 and competitor's antibody diluted at 1:250. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC |
| Clone | GT174 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Description | Centrifuge briefly prior to opening. |
| Immunogen | The immunogen used to generate this antibody corresponds to CA19-9/Sialyl Lewis A. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS, 20% Glycerol |
| Preservative | No Preservative |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: CA19-9/Sialyl Lewis A Antibody (GT174) [NBP3-13649] - CA19-9/Sialyl Lewis A antibody [GT174] detects CA19-9/Sialyl Lewis A protein at cell membrane by immunofluorescent analysis. Sample: SW1116 (high expression) and HT29 (low expression) cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CA19-9/Sialyl Lewis A stained by CA19-9/Sialyl Lewis A antibody [GT174] (NBP3-13649) diluted at 1:1000 and competitor's antibodydiluted at 1:1000. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/CA19-9-Sialyl-Lewis-A-Antibody-GT174-Immunocytochemistry-Immunofluorescence-NBP3-13649-img0001.jpg "Immunocytochemistry/Immunofluorescence: CA19-9/Sialyl Lewis A Antibody (GT174) [NBP3-13649] - CA19-9/Sialyl Lewis A antibody [GT174] detects CA19-9/Sialyl Lewis A protein at cell membrane by immunofluorescent analysis. Sample: SW1116 (high expression) and HT29 (low expression) cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CA19-9/Sialyl Lewis A stained by CA19-9/Sialyl Lewis A antibody [GT174] (NBP3-13649) diluted at 1:1000 and competitor's antibodydiluted at 1:1000. Blue: Fluoroshield with DAPI.")
![Flow Cytometry: CA19-9/Sialyl Lewis A Antibody (GT174) [NBP3-13649] - CA19-9/Sialyl Lewis A antibody [GT174] (NBP3-13649) detects CA19-9/Sialyl Lewis A by flow cytometry analysis. Sample: COLO 205 cell. Black: Unlabelled sample was used as a control. Red: CA19-9/Sialyl Lewis A antibody [GT174] (NBP3-13649) dilution: 1:50. Blue: Highly cited antibody [116-NS-19-9] dilution: 1:50. Acquisition of 20,000 events were collected for FACS analysis.](http://images.novusbio.com/fullsize/CA19-9-Sialyl-Lewis-A-Antibody-GT174-Flow-Cytometry-NBP3-13649-img0002.jpg "Flow Cytometry: CA19-9/Sialyl Lewis A Antibody (GT174) [NBP3-13649] - CA19-9/Sialyl Lewis A antibody [GT174] (NBP3-13649) detects CA19-9/Sialyl Lewis A by flow cytometry analysis. Sample: COLO 205 cell. Black: Unlabelled sample was used as a control. Red: CA19-9/Sialyl Lewis A antibody [GT174] (NBP3-13649) dilution: 1:50. Blue: Highly cited antibody [116-NS-19-9] dilution: 1:50. Acquisition of 20,000 events were collected for FACS analysis.")
![Sandwich ELISA: CA19-9/Sialyl Lewis A Antibody (GT174) [NBP3-13649] - Sandwich ELISA detection of Colo 205 and Jurkat whole cell extracts using CA19-9/Sialyl Lewis A antibody [GT174] (NBP3-13649) as capture antibody at concentration of 5 ug/mL and HRP-conjugated Ca19-9 antibody [GT8510] (NBP3-13652) as detection antibody at concentration of 1 ug/mL. Please notice that NBP3-13652 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-13649. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/CA19-9-Sialyl-Lewis-A-Antibody-GT174-Sandwich-ELISA-NBP3-13649-img0003.jpg "Sandwich ELISA: CA19-9/Sialyl Lewis A Antibody (GT174) [NBP3-13649] - Sandwich ELISA detection of Colo 205 and Jurkat whole cell extracts using CA19-9/Sialyl Lewis A antibody [GT174] (NBP3-13649) as capture antibody at concentration of 5 ug/mL and HRP-conjugated Ca19-9 antibody [GT8510] (NBP3-13652) as detection antibody at concentration of 1 ug/mL. Please notice that NBP3-13652 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-13649. Please contact us for custom HRP-conjugated antibody.")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")