C5b-9 Antibody (aE11) [Alexa Fluor™ Plus 680]

• Reactivity: Hu, Po, Ba, Eq, Pm
• Applications: ELISA, Flow, ICC/IF, IHC, WB (-)
• Clone: aE11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 680

C5b-9 Antibody (aE11) [Alexa Fluor™ Plus 680] Summary

• Immunogen: Purified C5b-9
• Epitope: Binds to a neoepitope exposed on C9.
• Specificity: Binds both membrane-bound MAC (active) and fluid-phase SC5b-9 complexes (inactive).
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Use in Bacteria reported in scientific literature (PMID:32900809).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for C5b-9 Antibody (aE11) [Alexa Fluor™ Plus 680]

• MAC
• Membrane attack complex
• sC5-b9
• sC5b-9
• TCC
• Terminal complement complex

Background

C5b-9 is also known as the terminal complement complex (TCC). The TCC consists of C5b, C6, C7, C8 and C9 and forms the membrane attack complex (MAC) as well as the non-lytic fluid-phase SC5b-9 complex (with protein S). The MAC forms channels in target cell membranes leading to cell lysis by osmotic leakage. The complexes contain neoantigens that are absent from the individual native components from which they are formed and this antibody is directed against a neoepitope exposed on C9 when incorporated into the TCC (1,2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.