BoNT-D Light Chain Antibody (695004) [Alexa Fluor® 350]

• Applications: WB
• Clone: 695004
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 350

BoNT-D Light Chain Antibody (695004) [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant C. botulinum BoNT-D Light Chain
  Thr2-Ser428
  Accession # P19321
• Specificity: Detects C. botulinum BoNT-D Light Chain in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant Light Chains of BoNT-A, -B, -C1, -E, -F, -G or recombinant Heavy Chains of BoNT-A,
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

BoNT-D LC (Botulinum neurotoxin serotype D light chain) is a 50-55 kDa member of the peptidase M27 family of molecules. It is the product of Clostridium botulinum, and inhibits acetylcholine release from neuromuscular junctions. This is accomplished by toxin internalization with subsequent cleavage of synaptobrevin, thus blocking synaptic vesicle fusion with the presynaptic membrane. Notably, BoNT-D is not toxic to human cells. BoNT/D precursor is 1276 amino acids (aa) in length. Following internalization and precursor proteolytic cleavage, it assumes a mature form that shows a disulfide-linked 442 aa light chain/enzyme N-terminus, and a 834 aa heavy chain/receptor-binding C-terminus. The 100 kDa heavy chain creates a channel within the endosome that allows for redox rupture of the disulfide bond and entry of the light chain into the cytosol. Covalent attachment of cargo molecules to the N-terminus is used to transport molecules into the cytosol. The D light chain shares less that 50% aa identity with the other botulinum serotype light chains.        

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.