BoNT-C1 Heavy Chain Antibody

• Reactivity: Ba-Cb
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

BoNT-C1 Heavy Chain Antibody Summary

• Immunogen: E. coli-derived recombinant BoNT-C1 Heavy Chain
  Asn866-Glu1291
  Accession # P18640
• Specificity: Detects C. botulinum BoNT-C1 Heavy Chain in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross‑reactivity with recombinant (r) BoNT‑D‑HCR is observed and 5% cross-reactivity with rBoNT-A-HCR, rBoNT-A-LC, and rBoNT‑B‑LC is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CbC4_4096
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL

Reactivity Notes

C. botulinum

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BoNT-C1 Heavy Chain Antibody

• BoNTC1 Heavy Chain
• BoNT-C1 Heavy Chain
• DcD

Background

BoNT-C1 (Botulinum neurotoxin C1 [heavy chain]) is a 100 kDa C-terminal fragment of the C1 Clostridum botulinum neurotoxin (holo)precursor. It constitutes a nonenzymatic part of a Zn²⁺-dependent M27 family endoprotease that potently blocks neurosecretion. The inactive 1291 amino acid (aa), 150 kDa C1 precursor is cleaved between Lys449Thr450 to generate a heterodimeric toxin held together by a preexisting interchain disulfide bond between Cys437 and Cys453. The 100 kDa C-terminus (heavy chain) acts as a ligand for neuronal membrane ecto-acceptors, inducing its translocation and internalization. The 50 kDa N-terminal light chain acts as an intracellular peptidase that cleaves SNAP-25 and syntaxin. This is suggested to block noradrenalin release from a readily releasable pool of vesicles. Over aa 866-1291, the C1 heavy chain shares less than 40% aa identity with the D-type neurotoxin heavy chain.                                  

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BoNT-C1 Heavy Chain Antibody (AF5425)(1)

Publication using AF5425

• Patrick M. McNutt, Edwin J. Vazquez-Cintron, Luis Tenezaca, Celinia A. Ondeck, Kyle E. Kelly, Mark Mangkhalakhili et al. Neuronal delivery of antibodies has therapeutic effects in animal models of botulism Science Translational Medicine 2021-01-06 [PMID: 33408188]

Bioinformatics

• Gene Symbol: CbC4_4096
• Uniprot:
  • P18640(C. botulinum)