BoNT-A Light Chain Antibody

• Reactivity: Ba-Cb
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

BoNT-A Light Chain Antibody Summary

• Immunogen: E. coli-derived recombinant Clostridium BoNT-A Light Chain
  Met1-Phe425
  Accession # Q45894
• Specificity: Detects C. botulinum BoNTA Light Chain in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant C. botulinum BoNTA Heavy Chain is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: atx
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL

Reactivity Notes

C. botulinum

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BoNT-A Light Chain Antibody

• BoNTA Light Chain
• BoNT-A Light Chain

Background

Botulinum neurotoxin type A is one of the seven serotypes of Botulinum Neurotoxins (BoNTs) produced by various strains of Clostridium botulinum (1, 2). BoNTs are synthesized as inactive single chain protein precursors and activated by proteolytic cleavage to generate disulfide-linked two-chain proteins. The 50 kDa light chain contains the catalytic domain, whereas the 100 kDa heavy chain contains an internal translocation domain and a receptor binding domain (3). BoNTs are the most potent protein toxins for humans. As zinc proteases, they cleave SNARE proteins to elicit flaccid paralysis in botulism by blocking acetylcholine release at the neuromuscular junction (2-4). E. coli-expressed recombinant light chains are active proteases. However, they are not toxic because they cannot enter into host cells in the absence of the heavy chains.

1. Willems, A. et al. (1993) Res. Microbiol. 144:547.
2. Montecucco, C. and Giampietro, S. (1993) Trends. Biochem. Sci. 18:324.
3. Turton, K., et al. (2002) Trends. Biochem. Sci. 27:552.
4. Schiavo, G. et al. (2000) Physiol. Rev. 80:717.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BoNT-A Light Chain Antibody (AF4489)(1)

Publication using AF4489

• Yeo, JC;Tay, FP;Bennion, R;Loss, O;Maignel, J;Pons, L;Foster, K;Beard, M;Bard, F; Botulinum toxin intoxication requires retrograde transport and membrane translocation at the ER in RenVM neurons eLife 2024-08-28 [PMID: 39196607] (Immunocytochemistry, Human)

Bioinformatics

• Gene Symbol: atx
• Uniprot:
  • Q45894(C. botulinum)