Biotin (Vitamin B7 or Vitamin H) Antibody (SPM375)


Immunohistochemistry-Paraffin: Biotin (Vitamin B7 or Vitamin H) Antibody (SPM375) [NBP2-32812] - Formalin-fixed, paraffin-embedded human Tonsil stained with biotinylated Lambda Light Chain probe followed by anit-biotin more

Product Details

Reactivity AllSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
0.2 mg/ml

Order Details

Biotin (Vitamin B7 or Vitamin H) Antibody (SPM375) Summary

Biotinylated sheep immunoglobulin
Depends on the target
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
IgG1 Kappa
Protein A or G purified
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  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 0.5-1ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • Western Blot 0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
Optimal dilution for a specific application should be determined.
Theoretical MW
244 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at 4C.
10 mM PBS with 0.05% BSA
0.05% Sodium Azide
0.2 mg/ml
Protein A or G purified


200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34771)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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