Biotin (Vitamin B7 or Vitamin H) Antibody (SPM375) [Alexa Fluor® 700]

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Product Details

Summary
Reactivity AllSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC-Fr, IHC-P
Clone
SPM375
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 700

Biotin (Vitamin B7 or Vitamin H) Antibody (SPM375) [Alexa Fluor® 700] Summary

Immunogen
Biotinylated sheep immunoglobulin
Localization
Depends on the target
Specificity
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Theoretical MW
244 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Biotin (Vitamin B7 or Vitamin H) Antibody (NBP2-34771AF700) (0)

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Secondary Antibodies

 

Isotype Controls

Additional Biotin (Vitamin B7 or Vitamin H) Products

Biotin (Vitamin B7 or Vitamin H) NBP2-34771AF700

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Contact Information

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Calculators

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