Biotin (Vitamin B7 or Vitamin H) Antibody (SPM375) [Alexa Fluor® 700]

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Product Details

Summary
Reactivity AllSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC-P
Clone
SPM375
Clonality
Monoclonal
Host
Mouse

Biotin (Vitamin B7 or Vitamin H) Antibody (SPM375) [Alexa Fluor® 700] Summary

Immunogen
Biotinylated sheep immunoglobulin
Localization
Depends on the target
Specificity
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Protein G purified
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Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
Alexa Fluor 700
Format
Alexa Fluor 700 (A=675-700, E=723)
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA 1-5ug/ml for coating
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Paraffin
Application Notes
Immunohistochemistry (No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.)
Theoretical MW
244 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Biotin (Vitamin B7 or Vitamin H) Antibody (NBP2-34771AF700) (0)

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Secondary Antibodies

 

Isotype Controls

Additional Biotin (Vitamin B7 or Vitamin H) Products

Biotin (Vitamin B7 or Vitamin H) NBP2-34771AF700

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