Biotin (Vitamin B7 or Vitamin H) Antibody (SPM375) [Alexa Fluor® 700]

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Product Details

Summary
Reactivity AllSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC-Fr, IHC-P
Clone
SPM375
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 700

Biotin (Vitamin B7 or Vitamin H) Antibody (SPM375) [Alexa Fluor® 700] Summary

Immunogen
Biotinylated sheep immunoglobulin
Localization
Depends on the target
Specificity
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Biotin (Vitamin B7 or Vitamin H) Antibody (NBP2-34771AF700) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NBP2-34771AF405
Alexa Fluor 488 NBP2-34771AF488
Alexa Fluor 647 NBP2-34771AF647
Alexa Fluor 700 NBP2-34771AF700
Allophycocyanin NBP2-34771APC
Biotin NBP2-34771B
DyLight 350 NBP2-34771UV
DyLight 405 NBP2-34771V
DyLight 488 NBP2-34771G
DyLight 550 NBP2-34771R
DyLight 650 NBP2-34771C
DyLight 680 NBP2-34771FR
DyLight 755 NBP2-34771IR
HRP NBP2-34771H
Janelia Fluor 549 NBP2-34771JF549
Janelia Fluor 646 NBP2-34771JF646
PE NBP2-34771PE
PerCP NBP2-34771PCP

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