Biotin (Vitamin B7 or Vitamin H) Antibody (Hyb-8) [Janelia Fluor® 646]


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Product Details

Reactivity AllSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Janelia Fluor 646

Order Details

Biotin (Vitamin B7 or Vitamin H) Antibody (Hyb-8) [Janelia Fluor® 646] Summary

Biotinylated sheep immunoglobulin
Depends on the target
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Protein G purified
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  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Store at 4C in the dark.
50mM Sodium Borate
0.05% Sodium Azide
Protein G purified


Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-34646AF350
Alexa Fluor 405 NBP2-34646AF405
Alexa Fluor 488 NBP2-34646AF488
Alexa Fluor 532 NBP2-34646AF532
Alexa Fluor 594 NBP2-34646AF594
Alexa Fluor 647 NBP2-34646AF647
Alexa Fluor 700 NBP2-34646AF700
Alexa Fluor 750 NBP2-34646AF750
Allophycocyanin NBP2-34646APC
Biotin NBP2-34646B
DyLight 350 NBP2-34646UV
DyLight 405 NBP2-34646V
DyLight 488 NBP2-34646G
DyLight 550 NBP2-34646R
DyLight 594 NBP2-34646DL594
DyLight 650 NBP2-34646C
DyLight 680 NBP2-34646FR
DyLight 755 NBP2-34646IR
FITC NBP2-34646F
HRP NBP2-34646H
Janelia Fluor 549 NBP2-34646JF549
Janelia Fluor 646 NBP2-34646JF646
PE NBP2-34646PE
PerCP NBP2-34646PCP

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